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Seasonal Changes in Serum Testosterone, LDH Concentration and Semen Characteristics in Markhoz Goats
Markhoz Goat, Semen Characteristics, Testosterone, Lactate Dehydrogenase
2016/5/9
This experiment was conducted to study variations of serum testosterone and seminal characteristics of Markhoz male goats. Blood samples were obtained via jugular vein, and semen was collected by usin...
内蒙古白绒山羊LDH-A基因cDNA的克隆与特性分析
内蒙古白绒山羊 LDH-A 基因克隆 表达模式分析
2009/12/3
【目的】克隆内蒙古白绒山羊乳酸脱氢酶A（LDH-A）基因并分析其基本表达模式。【方法】采用RT-PCR和3′ RACE技术克隆基因，将得到的基因cDNA序列及其编码的氨基酸序列进行生物信息学分析。利用RT-PCR方法进行组织表达检测。【结果】获得了内蒙古白绒山羊LDH-A基因全长cDNA序列。该基因cDNA全长1 649 bp，包含一个996 bp的ORF和642 bp 的3′ UTR。SMART...