搜索结果: 1-15 共查到“知识库 G6PD”相关记录17条 . 查询时间(0.045 秒)
胃癌间充质干细胞高表达G6PD促进肿瘤细胞迁移
间充质干细胞 胃癌 葡萄糖-6-磷酸脱氢酶 外泌体 迁移
2021/3/30
探究胃癌间充质干细胞(gastric cancer mesenchymal stem cells, GCMSCs)葡萄糖-6-磷酸脱氢酶(G6PD)的表达及其在肿瘤细胞迁移中的作用。方法?采用western blot检测G6PD在GCMSCs、骨髓间充质干细胞(bone marrow mesenchymal stem cells, BMMSCs)和胃癌细胞来源外泌体(exosome)预处理BMMS...
探讨G6PD对大肠癌HCT116和SW480细胞生长侵袭的影响，以及G6PD与HKⅡ在大肠癌及其癌旁上皮组织中的表达和两者的相关性。方法 将体外培养的大肠癌HCT116和SW480细胞进行G6PD过表达和干扰处理。Western blot法检测细胞中G6PD和HKⅡ的蛋白表达水平；流式细胞术分析细胞周期进程；葡萄糖氧化酶法检测培养液中葡萄糖的含量；划痕实验检测细胞侵袭能力；免疫组织化学法检测大肠癌...
G6PD缺陷与肠道病毒71型手足口病的关系
手足口病 葡萄糖-6-磷酸脱氢酶缺乏症 儿童
2014/11/17
探讨葡萄糖-6-磷酸脱氢酶(glucose-6-phosphate dehydrogenase, G6PD)缺陷对肠道病毒71型(Enterovirus 71, EV71)感染所致手足口病的影响及可能机制。方法 选取确诊为EV71感染手足口病的男性患儿220例,根据病情分为普通组(145例)和重症组(75例);将同期体检的132例健康男性儿童设为对照组。用全自动生化仪进行血液中G6PD、还原型谷胱...
目的观察高糖环境下THP-1细胞葡萄糖-6-磷酸脱氢酶（G6PD）活性及呼吸爆发功能改变及其可能信号通路。方法利用蛋白激酶A（PKA）特异性抑制剂PKI和PKA激动剂Forskolin 预处理不同浓度葡萄糖培养的THP-1 细胞，检测不同处理组的G6PD活性、腺苷酸环化酶(cAMP)含量、ROS产量及磷酸化p47phox的表达变化。结果与正常对照组相比，高糖组及PKA激动组的G6PD活性、ROS产...
Thalassemia,Iron and G6PD Deficiency in Lor Migrating Nomad Children,Southern Iran
Frequency Iron deficiency Thalassemia G6PD deficiency Nomads Iran
2015/9/21
Background: Ferropenia and consequent iron deficiency anemia (IDA), β-thalassemia, and glucose 6-phosphate dehydrogenase (G6PD) deficiency are three main common hematologic problems in Iran. This stud...
EFFECT OF FAVA BEAN EXTRACTS ON THE ERYTHROCYTE POTASSIUM TO HEMOGLOBIN RATION IN NORMAL SUBJECTS AND G6PD DEFICEICY PATIENTS
Fava beans in vitro toxicity fava beans
2009/12/29
A procedure for the in vitro evaluation of the toxicity of fava beans has been applied to 17 varieties of the bean and one batch of beans purchased on the open market; the latter consisted of unknown ...
目的:观察去氢表雄酮（DHEA）及G6PD基因的全硫代修饰反义寡核苷酸对体外培养Raji细胞的抑制作用。 方法:分别用不同浓度DHEA及G6PD反义寡核苷酸作用于体外培养Raji细胞，观察细胞存活、生长情况，同时采用逆转录-聚合酶链反应检测Raji细胞G6PD基因mRNA表达水平，测定G6PD活性。 结果:DHEA及G6PD反义寡核苷酸不影响Raji细胞存活，50 μmol/L、500μmol/L...
应用变性高效液相色谱法检测G6PD基因G392T及C1024T
葡糖磷酸脱氢酶缺乏 突变 基因
2009/8/5
目的： 通过对葡萄糖-6-磷酸脱氢酶（G6PD）基因两种已知突变的检测，观察变性高效液相色谱法(denaturing high performance liquid chromatography, DHPLC)的实用价值。 方法: 应用DHPLC方法及脱氧核糖核酸序列测定对葡萄糖-6-磷酸脱氢酶缺乏症患者和正常对照者进行已知突变的检测。 结果: 正常对照者第5、9-10外显子为单峰；G6PDG39...
1108 Turkish Cypriot men and 318 male labourers from mainland Turkey were screened for G6PD deficiency and haemoglobinopathy traits. The results revealed a 6.7% G6PD deficiency rate in the Turkish Cyp...
Glucose-6-Phosphate Dehydrogenase (G6PD) Variants in Three Minority Ethnic Groups in Central and Northern Vietnam
G6PD deficiency Vietnam G6PD Viangchan
2010/12/10
Community-based surveys for the detection of glucose-6-phosphate dehydogenase (G6PD) deficiency were conducted at three villages in central and northern Vietnam from 2001 to 2006. A total of 799 male ...
Glucose -6- Phosphate Dehydrogenase (G6PD) variants in East Sepik Province of Papua New Guinea: G6PD Jammu, G6PD Vanua Lava, and a novel variant (G6PD Dagua)
G6PD deficiency Papua New Guinea Dagua
2010/12/10
A community-based survey was conducted to assess the prevalence of G6PD deficiency and malaria in two villages (St. Martins, Dagua) of Wewak district, East Sepik Province, Papua New Guinea. A total of...
Glucose-6-phosphate dehydrogenase (G6PD) Deficiency
G6PD Oxidative damage Sex-linked inheritance Hemolytic anemia Mediterranean variant
2009/12/16
Glucose-6-phosphate dehydrogenase (G6PD) Deficiency is the most prevalent enzymopathy in mankind. It has sex-linked inheritance. This enzyme exists in all cells. G6PD deficiency increases the sen...
云南阿昌族G6PD基因突变G487A在DF213中的表达
阿昌族(AChang people) 葡萄糖-6-磷酸脱氢酶(Glucose-6-phosphate dehydrogenase) 基因(Gene) 表达载体(Expressional vector)
2008/3/6
为获得阿昌族G6PDWT和G6PDG487A重组蛋白，研究G6PDG487A的结构和功能改变，从云南省德宏州梁河县杞木寨乡湾中村阿昌族聚集地的G6PD缺陷家系先证者和正常阿昌族个体全血提取RNA，经RT-巢式PCR得cDNA, 将cDNA克隆至pMD18-T simple载体中并测序；错配碱基经定点突变修复后，目的基因亚克隆至pThioHis(A)载体，构建了阿昌族G6PD基因野生型和G4...
云南傣族中所见的G6PD突变型
基因突变 葡萄糖6磷酸脱氢酶缺乏症 G6PD突变型 聚合酶链反应
2008/1/9
利用错配碱基PCR/酶切法,在云南傣族中发现ntl388 G→A、ntl376 G→T和nt392 G→T G6PD基因突变型。其中主要为1388突变(18/23)。此3种突变也见于华南地区的汉族中,而有别于非中国人的突变型。提示傣族与汉族可能有同一民族渊源。利用PCR-SSCP方法在不同外显子中发现3例未知突变,待进一步DNA序列测定定型。