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The origin and mechanism of the secretion of membrane-bound particles in bovine seminal plasma were studied with transmission (TEM) and scanning (SEM) electron microscopy of the epididymis, vas defe...
The number of bovine spermatozoa separated in a swim-up procedure was quantified using an electronic cell counter. In an initial test of the swim-up procedure, non-frozen sperm samples with differen...
Purification of low Mr (less than 5000) inhibitors of FSH binding to receptors has been hampered by their low concentration in tissue extracts and physiologic fluids. The calf testis represents an a...
In vitro bovine cervical mucus (BCM) penetration tests, sperm penetration assays (SPA) using zona-free hamster eggs, and routine semen analyses were performed on a total of 136 freshly collected sem...
Since both adenosine and cAMP affect sperm motility and metabolism, the authors have studied the mode of interaction of adenosine with the adenylate cyclase activity in either the presence or the ab...
Penetration of spermatozoa in bovine cervical mucus (BCM) from two groups of men was investigated and correlated with the different parameters of spermograms from these same subjects. Sperm penetr...
A method is described for improving the quality of semen from subfertile men. Semen samples were filtered through bovine cervical mucus (collected at estrus) into spermatozoa-free seminal plasma (fr...
The activity of ornithine decarboxylase (ODC) was determined in primary cultures of bovine Sertoli cells after gonadotropin stimulation in vitro. A significant increase in both ODC activity and putr...
Plasma and semen concentrations of androgens and progestins were not influenced by repetitive ejaculation in bulls. Within 20 minutes after the injection of testosterone (100 mg Intravenously), plas...
Frozen bovine semen from males of well-established, generally high fertility levels was thawed and used in three experiments for in vitro capillary tube migration through cervical mucus. Bovine cerv...
Formation of a prefertilization sperm reservoir in mammals is thought to occur via sperm cell attachment to fallopian tube or oviduct epithelial cells (OEC). Recent data suggests that such an interact...
Bovine seminal platelet-activating factor acetylhydrolase was isolated to >90% purity in a single step using a butyl sepharose column. The procedure involves the elution of the activity by use of ...
The motility and velocity of bovine spermatozoa incubated in TCM-199 are reduced in comparison with those incubated in a simpler media made for sperm, such as modified Tyrode (Sp-Talp). Moreover, ...
Platelet-activating factor (PAF) is a potent lipid mediator that is inactivated by platelet-activating factor acetylhydrolase (PAF-AH). Platelet-activating factor bioactivity has been detected in ...
Poor motility and abnormal acrosomal morphology only partially explain the reduced fertility of cryopreserved bovine spermatozoa. To test the hypothesis that cryopreservation procedures (dilution,...

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