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New microscope technique reveals internal structure of live embryos(图)
New microscope technique internal structure live embryos
2017/9/4
University of Illinois researchers have developed a way to produce 3-D images of live embryos in cattle that could help determine embryo viability before in vitro fertilization in humans.Infertility c...
Green Light-emitting Diodes Light Stimuli during Incubation Enhances Posthatch Growth without Disrupting Normal Eye Development of Broiler Embryos and Hatchlings
Chick Embryo Incubation Light Emitting Diodes Light Stimuli
2016/11/10
Monochromatic green light-emitting diodes (LED) light stimuli influences the posthatch growth performance of chicks. This study was undertaken with the following objectives: i) to examine whether the ...
Single fixed-time laparoscopic intrauterine insemination as a tool to obtain low-diversity porcine embryos
laparoscopic insemination embryo diversity pig
2015/4/16
Double fixed-time insemination after ovulation induction is commonly used in pigs to obtain in vivo produced embryos at defined stages of development for downstream biotechnological applications. Howe...
Lower quality bovine embryos may be successfully used for sex determination
cattle embryo superovulation sex determination polymerase chain reaction embryo transfer
2015/3/13
The aim of this study was to evaluate the effect of sex determination procedures in Day 7 to Day 8 bovine embryos of various quality. For the purposes of comparison we used high quality (HQ) as well a...
The effect of vitrification in open pulled straws on pregnancy rates after transfer of in vivoproduced bovine embryos
bovine embryo superovulation cryopreservation freezing thawing vitrification embryo transfer
2015/3/5
The aim of this study was to compare pregnancy rates after transfer of in vivo produced embryos cryopreserved using open pulled straw (OPS) vitrification (Group V) or conventional freezing method as a...
Collection of oocytes from donors in the growth phase of follicular development can enhance the production of bovine embryos for cryopreservation
follicular development bovine oocyte in vitro embryos cryopreservation
2015/3/5
The present study was designed to compare the efficiency of bovine embryo production for cryopreservation between oocytes collected from donors in the growth phase of follicular development (GPFD) and...
Population of Circulating Primordial Germ Cells in Early Japanese Quail Embryos
primordial germ cells circulation erythrocytes quail embryo
2009/8/3
Avian primordial germ cells (PGCs) show a unique migration pathway during early development. As soon as the blood vessels have formed, PGCs enter the circulatory system, and migrate to the gonadal pri...
A Simple Method for Sexing Chicken Embryos at Stage X
blastodermal cell chicken embryo embryo culture PCR sexing
2009/7/31
A simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by...
Surface IgM-Inducing Factor in the Culture Supernatant of Bursal Epithelial Cells Derived from Chick Embryos
bursa of Fabricius epithelial cells sIgM-inducing factor
2009/7/31
Bursal epithelial cells derived from 14-day-old chick embryos were cultured under a serum-free condition and the recovered culture supernatant was analyzed for surface immunoglobulin M (sIgM)-inducing...
Genetic Factors Affect the Number of Circulating Primordial Germ Cells in Early Chick Embryos
primordial germ cells circulation chick embryo male female
2009/7/31
Avian primordial germ cells (PGCs) arise from the epiblast and circulate temporarily via the blood vascular system in their migration route to the gonadal anlage. There are egg-to-egg variations in th...
Culture of Chicken Embryos Obtained from the Anterior Region of the Magnum of the Oviduct after Removing a Thin Layer of Dense Albumen Capsule from the Ovum
chicken embryo culture dense albumen embryo manipulation fertilised ovum
2009/7/31
Fertilised ova, obtained from the anterior region of the magnum of the oviduct 60-80min after the preceding egg had been laid, were cultured in vitro with dense albumen adjusted to pH 7.0 after removi...
Effects of Soft X-Ray Irradiation to Stage X Blastoderm on Restriction of Proliferation of Primordial Germ Cells in Early Chicken Embryos
chick embryo primordial germ cell soft X-ray stage X blastoderm
2009/7/30
It is necessary to deplete the endogenous primordial germ cells (PGCs) from recipient embryos when germline chimaeric chickens are produced by the transfer of PGCs. In this study, effects of soft X-ra...
Intense Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Circulating Primordial Germ Cells in vitro and in vivo
gonads green fluorescent protein primordial germ cell transfection
2009/7/30
The present study was carried out to develop techniques for introducing exogenous DNA into primordial germ cells (PGCs) and expressing the introduced DNA efficiently in the gonads of developing chicke...
In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study
cattle in vitro embryos cryopreservation
2015/3/17
In this field study, embryos were derived from genetically highly valuable cows excluded from breeding due to reproductive disorders. Cows, 5 to 10 years old, of Czech Siemmental, Holstein Dairy and B...