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FASE刊“Large Animal Stem Cells”专辑综述文章全面阐述猪多能性干细胞最新研究进展及科学展望（图）
FASE刊 Large Animal Stem Cells 综述文章 猪 多能性干细胞 动物育种应用
2019/3/12
多能干细胞（PSC）因其具有自我更新和多向分化潜能，在体外可以长期培养并能进行基因编辑，其在生命科学与医学领域，以及动物育种应用等领域备受青睐。然而，大家畜，如猪，至今没有获得能够进行生殖嵌合、稳定传代、体外长期培养的多能干细胞系。这也是制约大家畜育种的瓶颈。自第一株小鼠胚胎干细胞报道以来，科学家对猪多能干细胞进了30多年的攻关，虽取得了良好的进展，但仍存在急需解决的科学问题。近日，由中国农业大学...
Retinoic acid attenuates oxidative injury in bovine mammary epithelial cells induced by hydrogen peroxide
vitamin A thioredoxin reductase oxidative injury
2017/12/25
The objective of this study was to explore how retinoic acid (RA) attenuates oxidative injury induced by hydrogen peroxide (H2O2) in bovine mammary epithelial cells (BMEC). Subconfluence BMEC were ran...
Retinoic Acid Attenuates Oxidative Injury in Bovine Mammary Epithelial Cells Induced by Hydrogen Peroxide
vitamin A thioredoxin reductase oxidative injury arachidonic acid p38 mitogen-activated protein kinase
2018/3/5
Jin L., Yan S., Shi B., Shi H., Guo X., Li J. (2017): Retinoic acid attenuates oxidative injury in bovine
mammary epithelial cells induced by hydrogen peroxide. Czech J. Anim. Sci., 62, 539–548.
The...
Effects of retinoic acid on the synthesis of selenoprotein and the antioxidative indices of bovine mammary epithelial cells in vitro
vitamin A antioxidant function dairy cows
2016/5/11
The present study was conducted to examine the effects of retinoic acid (RA) on the synthesis of selenoprotein and the antioxidative indices of bovine mammary epithelial cells (BMEC) in vitro and to e...
Dynamical Expression of MicroRNA-127-3p in Proliferating and Differentiating C2C12 Cells
MiR-127-3p C2C12 Proliferation Differentiation Goats
2016/12/28
MicroRNAs (miRNAs) are highly conserved, short non-coding RNAs that regulate gene expression at the posttranscriptional level. Although many miRNAs are identified in muscles and muscle cells, their in...
Effects of Extracellular Matrix Protein-derived Signaling on the Maintenance of the Undifferentiated State of Spermatogonial Stem Cells from Porcine Neonatal Testis
Porcine Spermatogonial Stem Cells Undifferentiation, Extracellular Matrix Proteins
2016/11/10
In general, the seminiferous tubule basement membrane (STBM), comprising laminin, collagen IV, perlecan, and entactin, plays an important role in self-renewal and spermatogenesis of spermatogonial ste...
Rapamycin Inhibits Expression of Elongation of Very-long-chain Fatty Acids 1 and Synthesis of Docosahexaenoic Acid in Bovine Mammary Epithelial Cells
Bovine Mammary Epithelial Cells Mammalian Target of Rapamycin Complex
2016/11/10
Mammalian target of rapamycin complex 1 (mTORC1) is a central regulator of cell growth and metabolism and is sufficient to induce specific metabolic processes, including de novo lipid biosynthesis. El...
Ramie Leaf Extracts Suppresses Adipogenic Differentiation in 3T3-L1 Cells and Pig Preadipocytes
Ramie Leaf Preadipocytes Proliferation Differentiation Obesity
2016/11/10
The present study was carried out to evaluate the anti-obesity effect of different concentrations of extracts of hot air-dried ramie leaf (HR) and freeze-dried ramie leaf (FR) in 3T3-L1 cells and pig ...
Isolation and Identification of Prepubertal Buffalo (Bubalus bubalis) Spermatogonial Stem Cells
Buffalo Spermatogonial Stem Cell Germ Cell Marker Proliferation
2016/11/10
Isolation and culture of spermatogonial stem cells (SSCs) are attractive for production of genetic modified offspring. In the present study, buffalo spermatogonial stem-like cells were isolated, cultu...
Production of Boer goat (Capra hircus) by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes
goat fibroblast clone
2015/6/8
The present study reports a birth of goat clone produced by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes. The donors of somatic cells were fibrob...
Effect of aromatase inhibitor (fadrozole) on proliferation, estradiol production and telomerase activity in pig granulosa cells in vitro
granulosa cells aromatase inhibitor proliferation
2015/6/8
The objective of the present work was to study the effect of a nonsteroidal aromatase inhibitor (fadrozole) on proliferation, estradiol production, aromatase expression and telomerase activity (TA) in...
Serum deprivation affects secretory activity of cultured porcine ovarian follicles and granulosa cells and their response to hormones
stress malnutrition IGF-I
2015/4/1
The aim of the present study is to understand the hormonal mechanisms of the effect of malnutrition on ovarian follicle functions. For this purpose, we examined the effect of malnutrition/serum depriv...
Differentiated Human Embryonic Stem Cells Enhance the In vitro and In vivo Developmental Potential of Mouse Preimplantation Embryos
Mouse Embryo d-hES Feeder Cell Co-culture Developmental Potential
2016/5/5
In differentiating human embryonic stem (d-hES) cells there are a number of types of cells which may secrete various nutrients and helpful materials for pre-implantation embryonic development. This st...
Gene expression of bovine cumulus oophorus cells after vitrification
cumulus oophorus gene expression in vitro maturation vitrification
2010/9/21
A challenge of cryobiology remains in to develop a method that provides adequate results of viability after cryopreservation of immature bovine oocytes. Vitrification has been the methodology that pro...
Effect of Mytomycin C Treatment on Feeder Cells and the Proliferation of Cultured Chicken Primordial Germ Cells Isolated from Embryonic Blood
chicken primordial germ cell culture feeder cell mytomycin C
2009/8/3
Chicken primordial germ cells isolated from embryonic blood were cultured in vitro on feeder cells derived from the gonads of 7-day incubated embryos. Primordial germ cells were proliferated about 13 ...