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土壤镰孢菌Real-Time QPCR定量方法的建立及应用
镰孢菌 实时荧光定量PCR 绝对定量
2015/9/28
基于镰孢菌ITS序列，设计一对应用于实时荧光定量PCR（Real-Time QPCR）反应的镰孢菌属特异性引物TS和TR，并建立了相应的Real-Time QPCR体系。应用该反应体系，绝对定量了无肥(NF)、化肥(NP)以及化肥配施有机肥(NPM)等 3 种施肥措施下大豆田土壤镰孢菌DNA含量。结果表明：引物TS和TR对镰孢菌属真菌有较好的特异性；Real-Time QPCR反应的扩增曲线中各梯...
Remote real-time monitoring soil water potential system based on GSM
Soil water potential real-time monitoring negative pressure meter
2009/3/4
Aiming at the limitation of traditional measuring soil water potential, the paper presents an information system based GSM to real-time monitor data coming from multiple data sources. The monitoring...