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P歟ni鑛a M.: Isolation and cryopreservation of early stages of germ cells of tench (Tinca tinca)
germ cells isolation transplantation
2015/7/2
A practical technique for isolation and cryopreservation of tench (Tinca tinca) (Cyprinidae,
Teleostei) early stages of germ cells (GC), including spermatogonia and spermatocytes, is reported for the...
The effect of probiotics potentiated with polyunsaturated fatty acids on the digestive tract of germ-free piglets
potentiated probiotics supplementation polyunsaturated fatty acids piglets
2015/3/13
Oil with higher a content of ω-3 polyunsaturated fatty acids (PUFA) was administered for 4 weeks to experimental germ-free piglets and from 21 days of age the piglets were inoculated perorally with pr...
Comparison of Rectal and Infrared Thermometry for Obtaining Body Temperature of Gnotobiotic Piglets in Conventional Portable Germ Free Facility
Gnotobiotic Piglet Rectal Temperature Infrared Thermometry Portable Germ Free Facility
2016/5/5
Gnotobiotic piglets (n = 10) were hand-reared in conventional germ-free facilities. Piglet body temperatures were measured with rectal and non-contact infrared thermometry (NIFT) on the lower eyelid, ...
超声波处理对小麦胚芽球蛋白理化和功能性质的影响（Effect of Ultrasound Treatment on Physicochemical and Functional Properties of Wheat Germ Globulin）
小麦胚芽球蛋白 理化性质 功能性质
2009/9/11
采用超声波处理小麦胚芽球蛋白以提高其功能性质。研究了超声波处理对小麦胚芽球蛋白理化和功能性质的影响。结果表明：经超声波处理后，小麦胚芽球蛋白的巯基和二硫键含量、紫外光谱和荧光光谱均发生了显著的变化。随着超声波功率的增加，小麦胚芽球蛋白的疏水性、起泡性、起泡稳定性、乳化性和乳化稳定性都降低；当超声波功率大于900?W时，由于小麦胚芽球蛋白重新伸展，疏水基团暴露增多，引起疏水性、起泡性、起泡稳定性、乳...
小麦胚芽的微波在线稳定化试验（Study on Technology of Stabilized Storage of Wheat Germ by On-line Microwave Processing）
麦胚 微波 在线 稳定化
2009/9/11
麦胚具有快速酸败变质的特性，为提高麦胚稳定性，利用研发的麦胚在线微波稳定化处理工艺和装备，选取不同微波功率和处理时间等工艺参数进行试验，研究不同条件对麦胚稳定化效果的影响。结果表明，采用微波功率2.8kW、时间4min处理的麦胚，在30d加速贮藏后，脂肪酶相对酶活17.63％，过氧化值3.81mmol/kg，酸价14.65mg/g，VE损失率5.29％，氮溶解指数51.10％，含水率3.72%，脂...
Effect of Mytomycin C Treatment on Feeder Cells and the Proliferation of Cultured Chicken Primordial Germ Cells Isolated from Embryonic Blood
chicken primordial germ cell culture feeder cell mytomycin C
2009/8/3
Chicken primordial germ cells isolated from embryonic blood were cultured in vitro on feeder cells derived from the gonads of 7-day incubated embryos. Primordial germ cells were proliferated about 13 ...
Identification of Transferred Chicken Germ Cells in Quail Gonad and Semen by Amplification of Chicken-Specific PCR Products
microsatellite DNA chicken PCR primordial germ cells quail
2009/8/3
A polymerase chain reaction (PCR) primer set for a chicken microsatellite locus on the Z chromosome, LEI0171 (GenBank Accession No.X85538), amplified a 363bp fragment of genomic DNA in chickens (Gallu...
Restoration of Genetic Resources from Ehime Native Chicken via Transferred Primordial Germ Cells (PGCs)
chimeras germ line primordial germ cell embryos
2009/8/3
This experiment was designed to produce chimeric chicken by transferring primordial germ cells (PGCs) of Ehime native chicken embryos to White Leghorn ones. Fertilized eggs produced by both chicken br...
Population of Circulating Primordial Germ Cells in Early Japanese Quail Embryos
primordial germ cells circulation erythrocytes quail embryo
2009/8/3
Avian primordial germ cells (PGCs) show a unique migration pathway during early development. As soon as the blood vessels have formed, PGCs enter the circulatory system, and migrate to the gonadal pri...
In vitro Proliferation of Chick Primordial Germ Cells Co-cultured with Germinal Ridge Stroma Cells
chicken PGCs in vitro culture proliferation germinal ridge stroma cells
2009/8/3
In order to develop the technique for in vitro culture of chicken primordial germ cells obtained from circulating embryonic blood vessels (cPGCs), participation of feeder cells and some of growth fact...
Developmental Genetic Analysis of the Avian Primordial Germ Cells and the Applications for Production of Chimeric Chickens
Germ Cells Chimeric Chickens Avian
2009/7/31
Developmental Genetic Analysis of the Avian Primordial Germ Cells and the Applications for Production of Chimeric Chickens.
Genetic Factors Affect the Number of Circulating Primordial Germ Cells in Early Chick Embryos
primordial germ cells circulation chick embryo male female
2009/7/31
Avian primordial germ cells (PGCs) arise from the epiblast and circulate temporarily via the blood vascular system in their migration route to the gonadal anlage. There are egg-to-egg variations in th...
Conservation of a Genetically Selected Broiler Line (42L)Using Cryopreserved Circulating Primordial Germ Cells (PGCS) Isolated by Filtration Method
Filtration method PGCs cryopreservation germ-line chimeras chicken
2009/7/31
A genetically selected broiler line (42L) developed at the Pennsylvania State University, PA, USA was used in the present study. In Experiment 1, embryonic blood was collected from chick embryos at ...
The Migratory Ability of Gonadal Germ Cells in the Domestic Chicken
germ cells migratory ability germline chimera chicken
2009/7/31
The migratory ability of germ cells collected from gonads at different stages of embryonic development was investigated in the domestic chicken.
In Experiment 1, primordial germ cells (PGCs) were col...
Effects of Ultraviolet Irradiation on the Migratory Ability of Primordial Germ Cells (PGCs) in the Domestic Chicken
UV irradiation PGCs Migratory ability embryo chicken
2009/7/31
The effect of ultraviolet (UV) irradiation on the ability of primordial germ cells (PGCs) to migrate toward the gonadal ridge was investigated in the domestic chicken.
When PGCs were irradiated with ...