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Transient GUS and GFP Expression in Spanish Red Cedar (Cedrela odorata L.) Somatic Embryos. Optimization of Bombardment Conditions and Evaluation of Selective Agent Lethal Dose
Biolistics Genetic Transformation Tree Genetic Modification Tropical Wood
2013/3/4
Cedrela odorata is a tropical tree widely appreciated for its wood. Commercial plantations are frequently hampered by the attack of the meliacea borer, Hypsipyla grandella, and the lack of resistant v...
α-硫辛酸对大豆农杆菌介导GUS瞬时表达和芽诱导的影响
大豆 α-硫辛酸 GUS瞬时表达 芽诱导率
2015/10/10
以适宜转化大豆受体YC-2为材料，研究了抗氧化剂α-硫辛酸对农杆菌介导GUS瞬时表达和芽诱导率的影响。结果表明：在共培养基中添加不同浓度的抗氧化剂可显著降低外植体的褐化率，随α-硫辛酸浓度的增加外植体褐化率明显减少，但白化率增加，且这些白化的外植体在后期不易产生诱导芽；共培养中添加不同浓度的α-硫辛酸可增强GUS瞬时表达率和芽诱导的再生率，且以25 mg?L-1效果最佳。
The Possible Final Localization of GUS (β-glucuronidase) Reporter Gene Product in Transgenic Plants is Vacuole
Gene targeting β-glucuronidase Neutral red Nicotiana tabacum Plasmolysis Reporter gene
2009/8/5
Localization of the product of a bacterium-originated reporter gene (β-glucuronidase: GUS) was studied using histochemical and electron-immunocytochemical methods in transgenic tobacco plants introdu...
为了验证大豆子房滴注转化方法的可重复性与遗传稳定性，将由报告基因GUS、表达调控序列（35S启动子，NOS终止子）和T-DNA边界序列3部分组成的基因表达框转入大豆。对T0代转化材料进行GUS组织化学染色分析和PCR检测。结果表明：在检测的340个幼胚中有12个呈GUS阳性，且在180个转化植株中有6个呈PCR阳性同时其叶片也呈GUS阳性，转化率分别为3.53%和3.33%。Southern杂交表...