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New CRISPR-based tool inserts large DNA sequences at desired sites in cells（图）
CRISPR 新型工具 大DNA序列 PAST 基因工程
2023/6/21
Building on the CRISPR gene-editing system, MIT researchers have designed a new tool that can snip out faulty genes and replace them with new ones, in a safer and more efficient way.
Within a given tissue or organ, cells may appear very similar or even identical. But at the molecular level, these cells can have small differences that lead to wide variations in their functions.
UVA Team finds way to view genes inside living cells,in real time
UVA Team view genes inside living cells real time
2017/4/27
For Mazhar Adli, the little glowing dots dancing about on the computer screen are nothing less than the fulfillment of a dream. Those fluorescent dots, moving in real time, are set to illuminate our u...
Researchers Use DNA‘Clews’to Shuttle CRISPR-Cas9 Gene-Editing Tool into Cells
Researchers DNA Clews Shuttle CRISPR-Cas9 Gene-Editing Tool
2015/9/8
CRISPR–Cas9 represents a promising platform for genome editing, yet means for its safe and efficient delivery remain to be fully realized. A novel vehicle that simultaneously delivers the Cas9 protein...
Myeloid cells induce neurofibromatosis type 1 aneurysm formation through inflammation and oxidative stress
Oxidative Stress cre/lox Murine Model Aneurysm
2015/5/25
Neurofibromatosis Type 1 (NF1) is a genetic disorder resulting from mutations in the NF1 tumor suppressor gene. Neurofibromin is the protein product of NF1 and functions as a negative regulator of Ras...
Low Levels of p53 Protein and Chromatin Silencing of p53 Target Genes Repress Apoptosis in Drosophila Endocycling Cells
apoptosis endocycling cells p53 protein
2015/1/7
Apoptotic cell death is an important response to genotoxic stress that prevents oncogenesis. It is known that tissues can differ in their apoptotic response, but molecular mechanisms are little unders...
Regulation of mammary stem/progenitor cells by p53 and parity
Biological sciences Progenitor cells Adult stem cells Breast neoplasms Li-fraumeni syndrome Mammary gland Notch proteins Tumor suppressor protein p53
2014/12/10
Breast cancer is the most common tumor among women with inherited mutations in the p53 gene (Li-Fraumeni syndrome). The tumors represent the basal-like subtype which has been suggested to originate fr...
催乳素和外源基因乳腺共表达载体可提高外源基因在哺乳细胞的表达Mammary Gland Dual－Expression Construct Containing Prolactin and Foreign DNAs Can Promote the Expression of Foreign Proteins in Mammary Cells
催乳素 乳铁蛋白 血小板生成素 表达
2008/1/8
摘要
为探讨应用牛催乳素促进乳腺生物反应器高效表达外源蛋白（如人乳铁蛋白、人血小板生成素）的可行性，构建两种牛催乳素和外源基因乳腺共表达载体。一种为牛催乳素基因和外源基因共用一个6.7 kb山羊β-酪蛋白启动子启动转录，两个基因以内部核糖体进入位点(IRES)连接；另一种为外源基因利用6.7 kb山羊β－酪蛋白启动子，催乳素基因利用2.0 kb山羊β-酪蛋白启动子，分别转录。脂质体转染后，RT...
氯化高铁血红素诱导K562细胞中β-珠蛋白基因表达的研究A Study of Inductive Effect of Hemin on Expression of theβ-globin Genes in K562 Cells
氯化高铁血红素 K562细胞 γ-珠蛋白基因 β-珠蛋白基因
2007/12/29
以绿色荧光蛋白（EGFP）基因为报道基因，构建了在β-珠蛋白启动子驱动及HS2元件调控下的重组表达载体HG，用脂质体转染法将其转染到K562细胞中，并用RT-PCR方法及流式细胞仪检测氯化高铁血红素(Hm)对K562细胞中β-珠蛋白基因表达及重组载体HG在K562细胞中瞬时表达的影响。结果显示：用30μmol/L Hm诱导K562细胞24、48及72h后，不仅其γ-珠蛋白mRNA水平升高，其β-珠...
Inhibition of respiratory syncytial virus replication in cultured cells by RNA-cleaving dnazyme
2007/7/28
专著信息
书名
Inhibition of respiratory syncytial virus replication in cultured cells by RNA-cleaving dnazyme
语种
英文
撰写或编译
作者
Zhao Changan,Zhao Xiaodong,Yu Haiguo
第一作者单位
出版社
中日医学大会
出版地
出版日期
2002年
月
日
标准书号
介质...
期刊信息
篇名
Presence of plastid and absence of mitochondrial DNA in male reproductive cells as evidence for cytoplasmic inheritance in Turnera ulmifolia and Zantedeschia aethiopica
语种
英文
撰写或编译
作者
吉栩,张泉,刘旸...
Induction effects of sulfur dioxide inhalation on chromosomal aberrationsin mouse bone marrow cells.
2007/7/28
期刊信息
篇名
Induction effects of sulfur dioxide inhalation on chromosomal aberrationsin mouse bone marrow cells.
语种
英文
撰写或编译
作者
Ziqiang Meng,Bo Zhang
第一作者单位
刊物名称
“Mutagenesis”,
页面
2002,17(3):215-217.
...
期刊信息
篇名
The Role of Short Telomeres in InducingPreferential Chromosomal Aberrations inHuman Ovarian Surface Epithelial Cells: ACombined Telomere Q-FISH andWhole-Chromosome Painting Study
语种
英文
撰写或编译
作...
期刊信息
篇名
Association of differentially expressed gene with activation of mouse hepatic stellate cells by high-density cDNA microarray.
语种
英文
撰写或编译
撰写
作者
5.Xiao-Jing Liu,Li Yang,Feng-Ming Luo,et al
第一作者...