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Analysis of Histone Lysine Methylation Using Mass Spectrometry
MudPIT histones transcription
2015/5/25
Histones are highly basic proteins which when digested by trypsin are hard to analyze using mass spectrometry. Because histones are basic nuclear proteins, a nuclei prep followed by acid extraction is...
LIM homeodomain 3 (LHX3) is an important regulator of pituitary and nervous system development. To date, twelve LHX3 gene mutations have been identified in patients with combined pituitary hormone def...
宫颈癌p16基因甲基化及表达的研究 Methylation and Aberrant Expression of the p16 Gene in Cervical Carcinoma
宫颈癌 p16基因表达 甲基化
2008/1/6
摘要
为了探讨p16基因甲基化及异常表达在宫颈癌中的意义，分别采用甲基化特异性PCR（MSP）方法检测不同病理类型和临床分期的60例宫颈癌组织中p16基因启动子区域5´CpG岛甲基化状态；采用PCR方法检测p16基因外显子1（E1)和外显子2(E2)纯合缺失情况;采用免疫组化的方法分析p16蛋白的表达缺失和减弱情况。结果显示正常对照组织及癌旁p16基因无甲基化，且无E1和E2缺失和p...