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Assessing the Importance of Sand as a Source of Fecal Indicator Bacteria (Escherichia coli and Enterococcus)
Escherichia coli Enterococcus
2015/7/21
The purpose of this activity is for students to investigate water quality, and the factors that affect it, at a local beach. This is accomplished by (1) measuring concentrations of Escherichia coli an...
Replication restart in Escherichia coli: Genetic studies at the interface of recombination and replication
DNA replication forks DNA damage proteins cells enzyme catalysis
2015/1/28
DNA replication forks are frequently detained during their progression from oriC to the replication terminus due to encounters with DNA lesions or proteins blocking the way. For survival, cells deploy...
Oxidative stress responses in Escherichia coli and Pseudomonas aeruginosa
Aerobic respiration reactive oxygen species ROS damage biological molecules cells detoxification oxidative stress transcription factors signal differential gene expression escherichia coli pseudomonas aeruginosa
2015/1/28
Aerobic respiration generates unavoidable reactive oxygen species (ROS, detrimental by-products that damage biological molecules. The accumulation of ROS beyond the cellular capacity for detoxificatio...
The SOS response in Escherichia coli: Single cell analysis using fluorescence microscopy
Biological sciences DNA repair RecA SOS response
2015/1/28
During the course of DNA replication, replication forks often stall or collapse as they proceed from oriC to the terminus due to housekeeping types of DNA damage or proteins bound to DNA. If the DNA i...
Reca Dynamics & the SOS Response in Escherichia Coli: Cellular Limitation of Inducing Filaments
DNA damage RadA RecA SOS response
2015/1/28
During the course of normal DNA replication, replication forks are constantly encountering "housekeeping" types of routine damage to the DNA template that may cause the forks to stall or collapse. One...
Differential afferent sensitivity to mucosal lipopolysaccharide from Salmonella typhimurium and Escherichia coli in the rat jejunum
5-HT afferent nerve enteritis hypersensitivity mesenteric
2014/5/16
Postinfectious irritable bowel syndrome may develop subsequent to acute bacterial enteritis. We therefore hypothesized that intestinal afferents may develop hypersensitivity upon exposure to luminal l...
大肠杆菌FC40系统静止期突变中的F因子转移The Occurrence of Actual F Factor Transfer during the Stationary-phase Mutation in Escherichia coli FC40
大肠杆菌FC40系统 适应性突变 F因子转移
2007/12/31
摘要本研究采用大肠杆菌GM133 rifr细胞和营养收集细胞HB214 strr进行适应性突变实验。在混合30min和2d 后添加链霉素杀死GM133基因型细胞，继续培养5d后，在选择平板上出现了一定数量的lac+strr基因型回复突变菌落。根据这些突变菌落的数量，估计在lac+突变产生之前，GM133和HB214细胞之间的接合频率分别为0.07%和7.47%。在培养了7d的选择平板上添加含链霉素...