搜索结果: 1-5 共查到“植物学 Isolation”相关记录5条 . 查询时间(0.062 秒)
改良CTAB法用于多年生植物组织基因组DNA的大量提取--An Efficient Macro-method of Genomic DNA Isolation from Actinidia chinensis Leaves
猕猴桃 DNA 提取 Southern杂交 Actinidia genomic DNA isolation Southern blot
2007/12/5
根据多年生植物组织富含多酚、多糖的具体特性,对现有的DNA提取方法进行了改进。通过增加提取缓冲液中b-巯基乙醇用量,简化氯仿/异戊醇抽提液步骤,改用经-20℃预冷异丙醇沉淀DNA等,对CTAB法加以改进。改进后方法具有以下优点:(1)获得的DNA质量良好,提取过程无明显的DNA降解,基本上排除了多酚物质的干扰;(2)用获得的DNA进行Southern杂交,可得到理想的杂交信号,可满足相关的分子研究...
Isolation of DNA for Sequence Analysis from Herbarium Material of Some Lichen Specimens
DNA isolation herbarium material lichen sequence analysis
2010/3/19
An improved protocol for the isolation of DNA from herbarium material of some lichen specimens is described. The isolated DNA is suitable for PCR reactions for DNA sequence analysis. The hexadecyltrim...
Isolation of Trichomes from Wheat and Other Species of Flowering Plants
Trichomes leaf wheat Triticum aestivum L. resistance vermin Oulema melanopiis L.
2010/3/18
Plant hairiness or pubescence as a specific phenotypical feature related to dehydration tolerance and resistance to leaf vermin is considered in this paper. A method for the isolation of trichomes, ea...
Isolation and Identification of Soilborne Fungi in Fields Irrigated by GAP in Harran Plain Using Two Isolation Methods
Hyphomycetes Microfungi Soil
2010/3/22
he microfungal flora of field soils irrigated by the South-eastern Anatolia Project (GAP: Güneydoğu Anadolu Projesi) in Harran Plain were investigated in terms of quality and quantity, using the ...
Protoplast Isolation in Lupin ( Lupinus mutabilis Sweet): Determination of Optimum Explant Sources and Isolation Conditions
Lupinus protoplast enzyme leaf mesophyll
2010/3/23
Effects of cultural factors on the yield, viability and division of protoplasts were investigated in Lupinus mutabilis Sweet containing a high protein content as well as a reasonable oil content which...