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Characterization of the Mitochondrial Proteome in Pyruvate Dehydrogenase Kinase 4 Wild-Type and Knockout Mice
Starvation Mitochondria Proteomics PDK4
2015/5/25
The goal of this study was to determine the effect of a PDK4 (pyruvate dehydrogenase kinase isoenzyme 4) knock-out on mitochondrial protein expression. A 2-D gel based mass spectrometry approach was u...
The role of pyruvate dehydrogenase kinase in glucose and ketone body metabolism
ketoacidosis hypothermia fatty acid pyruvate carboxylase
2015/5/25
The expression of pyruvate dehydrogenase kinase (PDK) 2 and 4 are increased in the fasted state to inactivate the pyruvate dehydrogenase complex (PDC) by phosphorylation to conserve substrates for glu...
Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities
Brilliant Cresyl Blue Embryonic Development Subcellular Characteristics
2016/5/19
The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogena...
Expression and processing of xanthin dehydrogenase/oxidase cDNA from African cape buffalo
Biological sciences Xanthin dehydrogenase Syncerus caffer Trypanosome Cape buffalo
2015/1/28
Our lab studies oxidative regulation in defense against extracellular pathogens, especially African Trypanosomes. ^ Trypanosomes are protozoan hemoflagellates. The organisms are transmitted by tsetse ...
Discovery of Novel Regulators of Aldehyde Dehydrogenase Isoenzymes
Aldehyde dehydrogenase high-throughput screening computational docking
2015/1/7
Over the past three years we have been involved in high-throughput screening in an effort to discover novel small molecular modulators of aldehyde dehydrogenase (ALDH) activity. In particular, we have...
Effects of Dietary Levels of Glycine, Threonine and Protein on Threonine Efficiency and Threonine Dehydrogenase Activity in Hepatic Mitochondria of Chicks
Threonine Dehydrogenase Threonine Efficiency
2016/5/16
This study was carried out to evaluate the relationship between threonine (Thr) efficiency and Thr dehydrogenase (TDG) activity as an indicator of Thr oxidation on chicks fed with levels of diets (CP ...
Effects of Dietary Protein and Threonine Supply on In vitro Liver Threonine Dehydrogenase Activity and Threonine Efficiency in Rat and Chicken
Threonine Oxidation Threonine Efficiency Threonine Dehydrogenase Liver of Rat and Chicken
2016/5/9
This study was conducted to assess the relation between threonine (Thr) oxidation rate and threonine efficiency on rat and chicken fed with graded levels of protein and threonine. The increase in thre...
Genotyping of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction
Genotyping Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes PCR-RFLP Dried Blood DNA Extraction
2010/9/20
We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genotyping method that works directly on whole human blood. This single-tube genotyping method wa...
Effect of Garlic Oil on Fatty Acid Accumulation and Glycerol-3-Phosphate Dehydrogenase Activity in Differentiating Adipocytes
3T3-L1 Adipocytes Fatty Acids Garlic Oil Glycerol-3-phosphate Dehydrogenase
2016/5/3
Garlic oil (GAR, Allium sativum L.) has been studied as a feed additive to improve animal production performance and decrease methane emission in ruminants. The present study was designed to determine...
Cloning and Sequence Analysis of Glyceraldehyde-3-Phosphate Dehydrogenase Gene in Yak
Yak Glyceraldehyde-3-Phosphate Dehydrogenase gene Cloning Housekeeping Gene
2016/4/28
In order to study the biological function of gapdh gene in yak, and prove whether the gapdh gene was a useful intra-reference gene that can be given an important role in molecular biology research of ...
乙醛脱氢酶2（ALDH2）基因研究进展及其与饮酒行为的关系Aldehyde Dehydrogenase (ALDH2) Polymorphism and Drinking Behavior*
乙醛脱氢酶2 多态性 饮酒行为
2007/12/7
摘要亚洲人群中普遍存在突变型的乙醛脱氢酶2（ALDH2*2）。此酶突变后活性缺失，导致乙醛在肝脏内大量累积使突变携带者在喝酒后会有脸红等不适反应，因此这可能影响他们的饮酒行为。由于ALDH2*2等位基因与饮酒行为相关，它也可能与酒精引起的肝脏损伤及某些癌症密切相关，而且，它在不同的亚洲人群中有不同的频率分布。近年来对ALDH2*2等位基因的序列结构、表达及其重要功能等有了更深入的了解，对ALDH2...
Screening and Characterization of Lactate Dehydrogenase-producing Microorganism
Lactobacillus Lactate Dehydrogenase Direct-fed Microbials
2016/4/18
The objective of this work was to isolate a microorganism, able to produce high lactate dehydrogenase (LDH) activity, for use as a microbial feed additive. The LDH is an important enzyme for lactate c...
The in Vitro Effects of Dexamethasone on Sheep Lens Glucose-6-Phosphate Dehydrogenase
Sheep eye lens dexamethasone glucose-6-phosphate dehydrogenase
2010/4/21
Glucose-6-phosphate dehydrogenase (G6PD) enzyme was purified from sheep lenses, and the in vitro effects of dexamethasone on the enzyme's activity were investigated. Sheep lens glucose-6-phosphate deh...
Purification and Characterization of Glucose 6-Phosphate Dehydrogenase from Sheep Liver
Glucose 6-phosphate dehydrogenase purification sheep liver
2010/4/21
Glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate: NADP+ oxidoreductase, EC 1.1.1.49; G6PD) was purified from sheep liver by a simple and rapid method. The purification process consisted of two...
Gene Cloning and Nucleotide Sequence of Human Dihydrolipoamide Dehydrogenase-Binding Protein
Pyruvate Dehydrogenase Complex Dihydrolipoamide Dehydrogenase-Binding Protein Nucleotide Sequence
2016/4/8
The pyruvate dehydrogenase complex (PDC), a member of -keto acid dehydrogenase complex, catalyzes the oxidative decarboxylation of pyruvate with the formation of CO2, acetyl-CoA, NADH, and H+....