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Creation of a Novel Hydrolase by Site-directed Mutagenesis of Malto-oligosyltrehalose Synthase
trehalose malto-oligosyltrehalose synthase site-directed mutagenesis transglycosylation hydrolysis
2008/4/20
Malto-oligosyltrehalose synthase (EC 5.4.99.15, MTSase) catalyzes the conversion of α-1,4-glucan to glycosyltrehalose by forming an α,α-1,1-glucosidic linkage on the reducing side of the α-1,4-glucan....