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Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants
Plant gene editing Fluorescent seed selection Co-transformation Cas9 zCas9i pEC1.2 PcUBi4-2
2023/11/30
Multiplex CRISPR-Cas9-based genome editing is an efficient method for targeted disruption of gene function in plants. Use of CRISPR-Cas9 has increased rapidly in recent years and is becoming a routine...
Nonhomologous end joining as key to CRISPR/Cas-mediated plant chromosome engineering
CRISPR Cas plant chromosome；plant breeding；Arabidopsis thaliana；Genome editing NHEJ
2023/11/30
Although clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas)-mediated gene editing has revolutionized biology and plant breeding, large-scale, heritable ...
CRISPR/Cas9-mediated P-CR domain-specific engineering of CESA4 heterodimerization capacity alters cell wall architecture and improves saccharification efficiency in poplar
cellulose；CESA4 homo/ heterodimerization；CRISPR/Cas9 Populus；saccharification secondary；cell wall
2023/11/30
Cellulose is the most abundant unique biopolymer in nature with widespread applications in bioenergy and high-value bioproducts. The large transmembrane-localized cellulose synthase (CESA) complexes (...
Covering the Combinatorial Design Space of Multiplex CRISPR/Cas Experiments in Plants
combinatorial gene knockout libraries crop breeding experimental design multiplex CRISPR/Cas screens plant genetic studies
2023/11/28
Over the past years, CRISPR/Cas-mediated genome editing has revolutionized plant genetic studies and crop breeding. Specifically, due to its ability to simultaneously target multiple genes, the multip...
Opportunities and challenges with CRISPR-Cas mediated homologous recombination based precise editing in plants and animals
CRISPR-Cas Homology directed repair (HDR) Non-homologous end joining (NHEJ) Knock-in Gemini virus replicon (GVR) Gene targeting (GT)
2023/11/16
The genome editing has revolutionized the agriculture and human therapeutic sectors by its ability to create precise, stable and predictable mutations in the genome. It depends upon targeted double-st...