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α-硫辛酸对大豆农杆菌介导GUS瞬时表达和芽诱导的影响
大豆 α-硫辛酸 GUS瞬时表达 芽诱导率
2015/10/10
以适宜转化大豆受体YC-2为材料，研究了抗氧化剂α-硫辛酸对农杆菌介导GUS瞬时表达和芽诱导率的影响。结果表明：在共培养基中添加不同浓度的抗氧化剂可显著降低外植体的褐化率，随α-硫辛酸浓度的增加外植体褐化率明显减少，但白化率增加，且这些白化的外植体在后期不易产生诱导芽；共培养中添加不同浓度的α-硫辛酸可增强GUS瞬时表达率和芽诱导的再生率，且以25 mg?L-1效果最佳。
The Possible Final Localization of GUS (β-glucuronidase) Reporter Gene Product in Transgenic Plants is Vacuole
Gene targeting β-glucuronidase Neutral red Nicotiana tabacum Plasmolysis Reporter gene
2009/8/5
Localization of the product of a bacterium-originated reporter gene (β-glucuronidase: GUS) was studied using histochemical and electron-immunocytochemical methods in transgenic tobacco plants introdu...