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Melatonin Attenuates Nitric Oxide Induced Oxidative Stress on Viability and Gene Expression in Bovine Oviduct Epithelial Cells, and Subsequently Increases Development of Bovine IVM/IVF Embryos
Melatonin Bovine Oviduct Epithelial Cell Bovine IVM/IVF Embryos Nitric Oxide Antioxidant
2016/5/6
The objective of the present study was to elucidate the fundamental mechanism of bovine oviduct epithelial cell (BOEC) co-culture on developmental capacity of bovine IVM/IVF embryos and to determine w...
Accurate prediction of gene expression by integration of DNA sequence statistics with detailed modeling of transcription regulation
gene expression DNA sequence statistics detailed modeling transcription regulation
2011/1/5
Gene regulation involves a hierarchy of events that extend from specific protein-DNA interactions to the combinatorial assembly of nucleoprotein complexes. The effects of DNA sequence on these process...
Mapping Dynamic Histone Acetylation Patterns to Gene Expression in Nanog-depleted Murine Embryonic Stem Cells
Mapping Dynamic Histone Acetylation Patterns Gene Expression in Nanog-depleted Murine Embryonic Stem Cells
2010/11/15
Embryonic stem cells (ESC) have the potential to self-renew indefinitely and to differentiate into any of the three germ layers. The molecular mechanisms for self-renewal, maintenance of pluripotency ...
Comparison of Gene Expression between Cumulus Oocyte Complexes and Naked Oocytes by Suppression Subtractive Hybridization in Swine
Suppression Subtractive Hybridization (SSH) Gene Expression Cumulus Cells Porcine
2016/5/3
In the antral follicle phase, several layers of cumulus cells surround the oocyte and play an important support and regulation role in oocyte development and maturation via intercellular communication...
上海交通大学医学院生物化学八年制课件 regulation of gene expression
上海交通大学医学院 生物化学 八年制 课件 regulation of gene expression
2009/10/13
上海交通大学医学院生物化学八年制课件 regulation of gene expression。
cDNA Microarray Analysis of Differential Gene Expression in Boar Testes during the Prepubertal Period
Boar Testis DNA Chip Analysis Gene Expression DNA Sequence
2016/4/29
In an attempt to understand the biochemical mechanism for the synthesis of the anabolic steroid, 19-nortestosterone, produced by prepubertal boar testes and its physiological role, normalized compleme...
Effects of Castration on Androgen Receptor, IGF-I Ea, MGF and Myostatin Gene Expression in Skeletal Muscles of Male Pigs
Pig Castration Skeletal Muscle Molecular Cloning Gene Expression
2016/4/29
Castration of male pig produces significant negative effects on skeletal muscle development. The androgen receptor (AR), two splice variants of insulin-like growth factor-I (IGF-I Ea and MGF) and the ...
Gene Expression Profiling of Liver and Mammary Tissues of Lactating Dairy Cows
Bovine Metabolism Microarray Gene Expression Profiling Liver Mammary Gland
2016/4/29
Gene expression profiling is a useful tool for identifying critical genes and pathways in metabolism. The objective of this study was to determine the major differences in the expression of genes asso...
Conjugated Linoleic Acid as a Key Regulator of Performance, Lipid Metabolism, Development, Stress and Immune Functions, and Gene Expression in Chickens
CLA Egg Meat Quality Lipid Composition Embryos Neonates Development Stress Immunity, Gene Expression
2016/4/29
It has been well documented from animal and human studies that conjugated linoleic acid (CLA) has numerous beneficial effects on health. In chickens, CLA exerts many effects on performance ranging fro...
Effect of Thyroid Hormone on the Gene Expression of Myostatin in Rat Skeletal Muscle
Thyroid Hormone Skeletal Muscle Hyperthyroidism Hypothyroidism Myostatin Expression
2016/4/29
Modification of thyroid hormone levels has a profound effect on skeletal muscle differentiation, predominantly through direct regulation involving thyroid hormone receptors. Nevertheless, little is kn...
Effects of Cell Status of Bovine Oviduct Epithelial Cell (BOEC) on the Development of Bovine IVM/IVF Embryos and Gene Expression in the BOEC Used or Not Used for the Embryo Culture
Bovine Oviduct Epithelial Cell Bovine IVM/IVF Embryos Growth Factor Gene Apoptosis Gene Antioxidant Gene
2016/4/28
The objective of this study was to investigate the effects of cell status of BOEC on development of bovine IVM/IVF embryos and gene expression in BOEC before or after culturing of embryos. The develop...
The Body Weight-related Differences of Leptin and Neuropeptide Y (NPY) Gene Expression in Pigs
Fat Deposition Gene Expression Leptin Neuropeptide Y Pig
2016/4/27
To determine if body weight change is directly related to altered leptin and neuropeptide Y (NPY) gene expression, we assessed adipose tissue weight, percent body fat, leptin and NPY mRNA levels and s...
Gene Microarray Analysis for Porcine Adipose Tissue: Comparison of Gene Expression between Chinese Xiang Pig and Large White
EST (Expressed Sequence Tag) cDNA Microarray Adipose Tissue Xiang Pig Large White SYBR Green Quantitative RT-PCR
2016/4/27
We created a cDNA microarray representing approximately 3,500 pig genes for functional genomic studies. The array elements were selected from 6,494 cDNA clones identified in a large-scale expressed se...
摘要 在动植物基因组中广泛存在一类非编码蛋白的RNA基因,产生长度大约为21~24个核苷酸的RNA,它们被命名为microRNA(miRNA)。 这是一类具有调节其他基因表达活性的小RNA。在生物的发育过程中发挥着重要作用。本文对这种基因表达调节途径的发现、机制功能及研究方法和现状作简要概述。
Abstract:Plant and animal genomes contain an abunda...
应用实时定量RT-PCR技术检测b地中海贫血珠蛋白基因的表达Quantitative Analysis of Human Globin Gene Expression in b-thalassemia Using Real-Time RT-PCR
地中海贫血/遗传学 珠蛋白基因 基因表达 实时定量RT-PCR
2008/1/6
摘要
为了定量检测 b 地中海贫血(b 地贫)的 a、b 和γ珠蛋白基因表达水平, 提取正常成人对照组、正常胎儿对照组和b 地贫患者组组成的样本 DNA,采用反向点杂交法(RDB)分析b 地贫各种突变类型;提取样本RNA用于进行针对a、b 和γ珠蛋白基因的荧光实时定量RT-PCR(FQ RT-PCR)。根据FQ RT-PCR原理,设计合成分别对应于a、b 和γ珠蛋白基因的3对引物和3条荧光探针,...