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中国林蛙与黑龙江林蛙血清蛋白比较分析
中国林娃 黑龙江林娃 血清蛋白 迁移率
2009/9/17
［目的］为林蛙种属的科学划分提供依据。［方法］以中国林娃和黑龙江林娃为材料，采用聚丙烯酰胺凝胶电泳法对其血清蛋白进行比较分析。［结果］从中国林蛙血清中共分离出25条蛋白带，从黑龙江林娃血清中共分离出23条蛋白带，其中迁移率为0.552、0.524、0.400、0.375、0.331、0.241、0.076、0.062、0.048的9条蛋白带为中国林蛙特有；迁移率为0.517、0.269、0.2...
冷应激对家禽神经内分泌系统的影响
冷应激 家禽 神经内分泌
2009/9/16
冷应激可引起家禽生产性能下降，诱发多种疾病，甚至造成死亡。研究家禽冷应激发生机制，探索科学的应激监测方法，对提高应激预防水平，保障畜牧业发展有重要意义。冷应激反应的生理机制几乎涉及所有器官和组织，能量代谢、神经内分泌、行为和生产性能等均发生改变，其中，神经内分泌在整合调节过程中起主导作用。主要综述了寒冷应激对以交感神经肾上腺髓质（SAM）系统、下丘脑垂体肾上腺皮质（HPA）轴和下丘脑垂体...
本研究旨在探讨锰对公鸡肝脏细胞色素P450酶系的影响。50日龄海兰褐公鸡400只，随机分为4组，分别在饲料中添加0、600、900、1 800 mg·kg1MnCl2建立亚慢性锰中毒模型，于30、60、90 d采取肝脏检测肝微粒体细胞色素P450酶系活性以及CYP2H1基因转录水平的变化。结果显示，细胞色素P450、b5含量，氨基吡啉N脱甲基酶(AND)和苯胺4羟化酶(AH)活性在各个时...
本研究旨在观察禁食是否对生长期北京鸭腺胃ghrelin免疫阳性细胞生成有影响。用免疫组织化学的方法检测25、35、50日龄的北京鸭（已经禁食72 h）单位面积（mm2）腺胃组织中ghrelin阳性细胞数目。研究发现在这3个时期，不管是禁食组还是自由采食组，大量ghrelin阳性细胞多分布于腺胃深层复管状腺中；禁食组腺胃中ghrelin阳性细胞数目比自由采食组极显著增加（P＜0.01）。结果提示：禁...
为了探讨小型猪复合麻醉剂对小型猪血流动力学的影响及其作用机制，选用14头中国实验用小型猪肌肉注射小型猪复合麻醉剂（0.15 mL·kg1），并在注药前及注射药后5、10、30、45、60、80、100、120 min进行无创血压、心率（HR）的监测；并同步采取前腔静脉血样，采用放免法检测肾素（PRA）、血管紧张素（AⅡ）和醛固酮（ALD）活性/含量。试验结果表明：无创血压、HR主要是在注药后5~...
5-Aza诱导BMSC分化为心肌细胞的分子生物学检测
骨髓间充质干细胞 5-氮杂胞苷 心肌细胞
2009/8/31
【目的】利用分子生物学方法探讨5-氮杂胞苷（5-azacytidine，5-Aza）体外诱导牛骨髓间充质干细胞（bone marrow stromal cells，BMSC）向心肌细胞的分化，为转基因良种肉牛培育提供种子细胞，提高转基因效率。【方法】利用获取的纯化稳定的P4、P8、P12代BMSC各经5、10、15μmol?L-1的5-Aza进行体外诱导分化，统计并计算心肌细胞的转化率，对诱导出现...
Actigraph as a Device for Estimation of Heat Production of White Leghorn Hens under High Ambient Temperature
heat production activity count standing time ambient temperature feed intake
2009/8/3
Eight commercial Single Comb White Leghorn hens were used to study the effects of high ambient temperature on behavioral activities and to select a better parameter for the estimation of heat producti...
Innervation Pattern of Acetylcholinesterase (AChE)-Positive Nerves in the Internal Carotid Artery and Cerebral Arterial Tree of the Pigeon
acetylcholinesterase-positive nerves internal carotid artery cerebral arterial tree pigeon
2009/8/3
The pathway and distribution of nerves positive for acetylcholinesterase (AChE) in the internal carotid artery (ICA) and cerebral arterial tree were investigated histochemically in the pigeon. In this...
Intracerebroventricular Injection of Corticotropin-Releasing Factor Does not Alter Monoamine Content of the Paraventricular Nucleus of the Hypothalamus in Neonatal Chicks
corticotropin-releasing factor (CRF), monoamines, paraventricular nucleus of the hypothalamus (PVN), chicks
2009/7/31
Intracerebroventricular (i.c.v.) injection of corticotropin-releasing factor (CRF) on monoamine contents of the paraventricular nucleus of the hypothalamus (PVN) was investigated in neonatal chicks. T...
Effects of Heat Stress on Histological Features in Pituicytes and Hepatocytes,and Enzyme Activities of Liver and Blood Plasma in Japanese quail (Coturnix japonica)
heat stress pituicyte hepatocyte enzyme activities Japanese quail
2009/7/31
Two experiments were conducted to ascertain the histological features in pituicytes and hepatocytes and the enzyme activities of liver and blood plasma at 8 weeks of age in Japanese quail (Coturnix ja...
Effect of Physical Activities on Heat Production of White Leghorn Hens under Different Lighting Regimes
heat production activity count standing time lighting regime laying hen
2009/7/31
Six Single Comb White Leghorn laying hens (Shaver, Star cross 288) were used to estimate heat production (HP) for physical activities under different lighting regimes (LR) of 12L : 12D, 14L : 10D and ...
Correlating Changes in Follicular VLDL Binding and Follicular Aromatase Activity in the Ovulation Cycle of the Laying Hen
Ovarian follicle VLDL-receptor Aromatase Ovulation cycle
2009/7/31
We examined whether very low-density lipoprotein receptors (VLDLR) on the oocyte membrane contributed to changes in VLDL uptake into the yolk by the ovarian follicle during the ovulation cycle of the ...
Changes in Thickness and Weight of Shell Portion of Egg and in Weight and Size of Whole Egg during the Stay in the Uterine Part of the Oviduct (Shell Gland) in the Guineafowl:Comparison with White Leghorn Hens
guineafowl shell gland eggshell formation egg size
2009/7/31
The thickness and weight of the shell portion (shell with shell membrane) of eggs at various times during the stay in the uterus and at oviposition were found to be greater in the guineafowl than thos...
Time Course Changes in 13C Enrichment of Respiratory CO2 in Chickens Intravenously Single-Injected with [1-13C] Sodium Acetate
oxidation intravenous acetate 13C enrichment chicks
2009/7/31
Time course changes in 13C enrichment of respiratory CO2 in young chickens intravenously injected with [1-13C] sodium acetate were examined to clarify the rapidity of oxidation of intravenous acetate...
Effects of Amino Acid,Enzyme Mixture and Phytase Added to Low Protein and Low Phosphorus Diet on Performance and Excretion of Nitrogen and Phosphorus in Broilers
broiler excretion of nitrogen excretion of phosphorus amino acid phytase
2009/7/31
In the purpose to reducing excretion of nitrogen (N) and phosphorus (P) without the influence of the growth performances and abdominal fat in broilers, a feeding trial and balance test were conducted....