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Human sperm mitochondrial function related to motility：a flow and image cytometric assessment
Human sperm mitochondrial function motility cytometric assessment
2009/2/23
Current evaluation of male fertility, routinely estimated by sperm count, motility, and morphology, provides only crude information about the fertility state of individuals. Both flow and image cyto...
The effect of tumor necrosis factor on human sperm motility in vitro
tumor necrosis factor human sperm motility
2009/2/23
Tumor necrosis factor (TNF alpha) is present in elevated levels in peritoneal fluid from infertile women with endometriosis. The effect of TNF alpha on human sperm motility in vitro was evaluated ...
Synchronous assay for human sperm capacitation and the acrosome reaction
human sperm capacitation acrosome reaction
2009/2/23
A synchronous acrosome reaction system was established for human spermatozoa. Seminal plasma is removed from the spermatozoa by centrifugation and the washed spermatozoa are capacitated in a modif...
Evaluation of computerized analysis of sperm movement characteristics and differential sperm tail swelling patterns in predicting human sperm in vitro fertilizing capacity
sperm movement characteristics differential sperm tail predicting human sperm
2009/2/23
Multivariate discriminant analysis was used to evaluate the usefulness of computer image analysis of sperm movement characteristics and differential patterns of sperm tail swelling after hypoosmot...
Relationship of human sperm acrosin and proacrosin to semen parameters. II. Correlations
human sperm acrosin semen parameters
2009/2/23
Previous results, simultaneously confirmed by others, suggest that a relationship exists between sperm acrosin levels and fertility in man. The assessment of sperm acrosin may therefore be a useful ...
Correlation between in vitro fertilization and human sperm density and motility
Correlation fertilization human sperm density motility
2009/2/23
The conventional sperm characteristics of density (millions per milliliter) and motility, scored in a semi-subjective way, were correlated with results of an on-going in vitro fertilization and em...
Improvement in the quality and fertilization potential of a human sperm population using the rise technique
fertilization potential human sperm population rise technique
2009/2/23
Semen from 63 individuals participating in an in vitro fertilization program was processed using a modified rise technique. Overall normal morphology was significantly improved in the rise (79.2%) as ...
Correlation between human sperm swelling in hypoosmotic medium（hypoosmotic swelling test）and in vitro fertilization
human sperm hypoosmotic medium（hypoosmotic swelling test） vitro fertilization
2009/2/23
Human ejaculates (n = 83) were analyzed for standard sperm parameters (concentration, motility, and morphology), as well as for the ability of the spermatozoa to react (swell) in a hypoosmotic mediu...
Analysis of human sperm function following exposure to the ionophore A23187. Comparison of normospermic and oligozoospermic men
A23187 sperm motility fertilization ohgozoospermia
2009/2/20
Time exposure photomicrography and interspecific in vitro fertilization techniques have been used to compare the responses to the divalent cation ionophore A23187 of spermatozoa from normal fertile an...
Human sperm penetration in bovine cervical mucus. Clinical studies I
Human sperm penetration bovine cervical mucus
2009/2/19
Penetration of spermatozoa in bovine cervical mucus (BCM) from two groups of men was investigated and correlated with the different parameters of spermograms from these same subjects. Sperm penetr...
Ultrastructural Observations on the Penetration of Human Sperm into the Zona Pellucida of the Human Egg In Vitro
ultrastructure zona pellucida human ova acrosome reaction
2009/2/19
Sperm penetration into the zona of 46 human eggs was examined by electron microscopy. Preovulatory oocytes were aspirated at laparoscopy and inseminated by methods which produced normal pregnancie...
A Competitive In Vitro Assay of Human Sperm Fertilizing Ability Utilizing Contrasting Fluorescent Sperm Markers
Spermatozoa fertilization sperm motility
2009/2/19
Fluorescein isothiocyanate (FITC) and tetramethyl rhodamine isothiocyanate (TRITC) were evaluated for use as contrasting fluorescent labels on living human spermatozoa. Unlabeled (control), FITC...
Relationship of Human Sperm Acrosin and Proacrosin to Semen Parameters. I. Comparisons Between Symptomatic Men of Infertile Couples and Asymptomatic Men, and Between Different Split Ejaculate Fractions
acrosin proacrosin semen spermatozoa split ejaculate fertility infertility
2009/2/19
Acrosin is a sperm serine proteinase whose activity appears to be essential for fertilization in mammals, although this has not been shown in man due to experimental limitations. The present stu...
Evaluation of oxidative DNA damage in human sperm and its association with male infertility
8-Hydroxydeoxyguanosine spermatozoa infertility
2009/2/18
Recently, there is increasing evidence suggesting that oxidative sperm DNA damage is closely associated with impaired sperm function and male infertility. 8-Hydroxydeoxyguanosine (8-OHdG) is consi...
Increased in vitro binding of fresh and frozen-thawed human sperm exposed to a synthetic peptide
Profertility peptide sperm-egg binding human
2009/2/18
Prosaposin is a well-characterized, approximately 68-kDa protein found in many tissues and as a normal component of human semen. A fragment of prosaposin apparently is involved in primary sperm-eg...