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Towards the isolation of esterase and amidase catalytic antibodies: Examination of germinal center diversity by cloning and sequence analysis of genes encoding monoclonal antibodies elicited by a transition state analogue
Esterase catalytic antibodies amidase amide ester hydrolysis reaction hybrid cells B cells the TSA induced antibodies genes cells cell lines
2014/12/11
In this study, a transition state analogue (TSA) approach was employed for the isolation of catalytic antibodies with esterase or amidase activity. The TSA used in this study, phosphonamidate, mimics ...
Effects of substrate and separation method on acrosin amidase measurements
Enzyme activity DL-BAPNA L-BAPNA swim-up
2009/2/16
The purpose of this study was to increase the accuracy and reproducibility of the acrosin amidase assay and to assess the effects of different methods of sperm isolation on total sperm acrosin act...
An assay for sperm amidase activity has been modified so that many samples can be measured rapidly, making it more suitable for use in a clinical setting. The modified assay gave the same results ...
High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium
penicillin G gene
2006/12/5
28 November 2006,During the last years B. megaterium was continuously developed as production host for the secretion of proteins into the growth medium. Here, recombinant production and export of B. m...