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Proteomic profiling of salivary gland after nonviral gene transfer mediated by conventional plasmids and minicircles
drug development toxicology translational research
2014/11/6
In this study, we compared gene transfer efficiency and host response to ultrasound-assisted, nonviral gene transfer with a conventional plasmid and a minicircle vector in the submandibular salivary g...
Construction of Prostate-Specific Expressed Recombinant Plasmids With High Transcriptional Activity of Prostate-Specific Membrane Antigen（PSMA）Promoter/Enhancer
Adenocarcinoma regulatory element
2008/12/23
To screen different combinations of prostate-specific membrane antigen (PSMA) promoter/enhancer with the strongest transcriptional activity in prostate-specific cells, we used PSMA regulatory elemen...
摘要
为了用绿色荧光蛋白标记观察人类无精症相关基因ZNF230在Cos7细胞中的蛋白质表达及定位，用PCR方法扩增得到突变的人和小鼠mt-ZNF230和mt-znf230基因，使其3′端的终止密码TGA突变为TGG，并装入T-载体，双酶切后通过定向克隆将其与真核表达载体pEGFP-N1的绿色荧光蛋白（green fluorescence protein，GFP）基因融合，构建了ZNF230—荧光...