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Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium
18S gene GP60 gene ethidium bromide GelRedTM
2015/4/16
Differentiating between the Cryptosporidium species and their subtypes using only microscopy is impossible. Therefore, molecular tools are indispensable for accurate species and subtype diagnosis. How...
Genotyping of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction
Genotyping Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes PCR-RFLP Dried Blood DNA Extraction
2010/9/20
We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genotyping method that works directly on whole human blood. This single-tube genotyping method wa...
DNA extraction from human saliva deposited on skin and its use in forensic identification procedures
DNA Forensic dentistry Saliva
2009/10/20
Saliva is usually deposited in bite marks found in many homicides, assault and other criminal cases. In the present study, saliva obtained from volunteers was deposited on skin and recovered for DNA e...
Skim Milk Drastically Improves the Efficacy of DNA Extraction from Andisol,a Volcanic Ash Soil
environmental DNA microbial community analysis molecular methods unculturable microorganisms
2009/9/9
There is an increasing interest in the extraction of DNA from soil, since molecular techniques facilitate the analysis of the microbial community including unculturable microorganisms. Though various ...
Simplified DNA Extraction Methods for Sexing Chick Embryos
DNA extraction sexing PCR XhoI repetitive sequence embryo chicken
2009/7/31
We investigated the efficiency of DNA extraction and the accuracy of polymerase chain reaction- (PCR-) based sexing of DNA that was obtained from adult chickens and early chicken embryos. DNA samples ...
A Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken Embryos
alkaline chicken DNA extraction feather sexing
2009/7/29
The objective of this study was to develop a simple and quick DNA extraction procedure for rapid diagnosis of sex of chicken and its embryos using multiplex polymerase chain reaction (PCR). Using alka...
Evaluation of the Efficacy of Five DNA Extraction Methods for the Detection of Mycobacterium tuberculosis DNA in Direct and Processed Sputum by an In-House PCR Method
DNA extraction Mycobacterium tuberculosis PCR
2009/5/20
Aim: DNA extraction is an important step from clinical samples for molecular diagnosis of tuberculosis by PCR. The aim of this study was to evaluate the efficacy of five DNA extraction methods [boil...
A Simplified Rice DNA Extraction Protocol for PCR Analysis
DNA extraction rice polymerase chain reaction molecular marker simple sequence repeats
2009/1/7
A simple protocol was established for DNA extraction using etiolated rice seedlings, whereby rice DNA was directly extracted in 0.5 mol/L NaOH solution in a single eppendorf tube. Results of comparati...
DNA提取的应用与相关技术分析Application of DNA Extraction and Analysis of Its Influence Factors
DNA提取 血液 新鲜组织 石蜡标本 冰冻组织
2007/12/30
摘要为了分析影响提取DNA的有关因素，采用标准酚—氯仿抽提法和蛋白酶消化法提取DNA。结果表明，平均每mL全血可提取200 ~ 300μgDNA；新鲜组织及OCT包埋冰冻组织提取DNA，平均每0.2g组织可获得200~300μgDNA；直接将石蜡标本提取物制作模版，PCR扩增良好。从4种组织中均获得较为纯净的DNA；OCT对组织DNA无不良影响。
Abstract:To explore infl...
期刊信息
篇名
An efficient protocol for genomic DNA extraction from citrus species
语种
英文
撰写或编译
作者
Cheng YJ,Guo WW,Yi HL,Pang XM,Deng XX
第一作者单位
刊物名称
Plant Molecular Biology Reporter
页面
21(2): 177-178 (2003
出...