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Spirodela polyrhiza belongs to the family Lemnaceae (duckweed), which is a group of small aquatic plants offering an attractive plant expression system for the production of recombinant protein. No fr...
The present study describes a reliable method for callus induction of Catalpa bungei plant. Calli were in vitro initiated from leaves, stem and petiole segments using DKW basal media. The effect of co...
An efficient and reproducible protocol has been developed for the in vitro production of an endangered medicinal climber Tylophora indica (Burm.f.) Merr. via leaf explants. Different types of calli pr...
The effect of plant growth regulators (PGRs) and carbohydrate sources on callus induction, callus growth, and plant regeneration in foxglove was examined. Explants were transferred onto MS medium with...
The bark of Magnolia obovata has been used as a source of traditional Japanese herbal medication because it contains the medicinal ingredient such as magnolol, which is a kind of neolignan. In this st...
Potato extract (PE),corn extract (CE) and papaya extract (PAE) at concentrations of 25, 50 and 100 mL L–1enhanced callus growth on New Phalaenopsis (NP) medium containing sucrose. Among the various co...
Soy bean (Glycine max L.) callus line tolerant to 9% Fusarium oxysporum metabolite was selected. Initiation and maintenance of callus as well as selection process was carried out on Murashige and Sko...
Histological characteristics of globular shaped pollen callus (globules) developed from multicellular pollen grains were examined in rice anther culture (Oryza sativa L.). The ultrastructure of polle...
Morphological details of rice callus cultures were examined at the initial stage of plantlet development, together with the growth of callus established from mature seeds, with the aid of scanning elc...
The formation of pollen callus was induced when rice anthers, which contained microspores in the mid-uninucleate and late-uninucleate stage, were cultured. The ultrastructure of the microspores was al...
The scanning electron microscopy-light microscopy method (SEM-LM), routinely prepared SEM specimens can be reprocessed for light microscopy (LM), is presented. In this method, specimens were embedded ...
High yield of protoplast was obtained from the green callus tissue of finger millet at one week after subculture on MS medium with 0.2 mgl-1 2, 4-D and 0.1 mgl-1 kinetin. The protoplasts were release...
Ultrastructural observations were undertaken to study the viability of fusion products by electrofusing the protoplasts from the mesophyll cells of pansy leaves and the cultured callus cells of wild v...
Callus was initiated from mature seeds without the rinds of barnyard grass, Echinochloa oryzicola Vasing, on a modified Murashige and Skoog (MS) medium containing 2.0∼20.0 mgl-1 2, 4-D. The rate...
High yields of viable protoplasts were obtained from leaf mesophyll of 10-day-old peanut seedlings (Arachis hypogaea L. var. Lokal). An enzyme solution containing 1% Cellulase YC, 2% Meicelase P-1, 0....

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