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Measurement of seminal LDH-X and transferrin in normal and infertile men
seminal LDH-X transferrin normal and infertile men
2009/2/23
LDH-X, an isoenzyme of lactate dehydrogenase specific for germinal epithelium activity, has been measured in the seminal plasma of infertile subjects whose infertility had different origins. In the ...
Measurement of prostate-specific antigen and human glandular kallikrein 2 in different body fluids
prostate-specific antigen human glandular kallikrein 2 body fluids
2009/2/18
It has been demonstrated that prostate-specific antigen (PSA), in spite of its name, can be detected in body fluids and tumors from a variety of organs. Investigations have shown that human glandu...
Measurement of immunoglobulin G levels in adult rat testicular interstitial fluid and serum
immunoglobulin G levels adult rat serum
2009/2/13
Total immunoglobulin G (IgG) in rat testicular interstitial fluid and serum was measured by two-site enzyme-linked immunosorbent assay, which displayed minimal cross-reactivity with IgA or IgM. Norm...
Measurement of human sperm intracellular water volume by electron spin resonance
Sperm volume chromium oxalate spin label tempone toxicity
2009/2/11
An electron spin resonance technique using the spin label tempone and the broadening agent potassium chromium oxalate was used to measure the water volume of human sperm. The toxicity of tempone (...
Is an immunoassay available for the measurement of bioactive LH in serum?
LH immunoassay bioassay
2009/2/10
An in vitro bioassay for luteinizing hormone (LH) is in our opinion the "gold standard" bioassay. The rodent interstitial cell testosterone assay (RICT) is specific for bioactive LH and very sensitive...
Measurement of bovine sperm nuclear shape using Fourier harmonic amplitudes
bovine sperm nuclear shape
2008/12/29
An objective method for measuring bovine sperm nuclear shape was developed. Digital images of bovine sperm stained with propidium iodide were collected and Fourier functions used to describe the per...
Quality control of reactive oxygen species measurement by luminol-dependent chemiluminescence assay
Semen spermatozoa luminol oxidative stress male
2008/12/29
A total of 28 donor semen samples were used to evaluate the characteristics of laboratory variability in measuring reactive oxygen species (ROS). The objectives of this study were to assess the intera...
Measurement of intracellular calcium concentration and plasma membrane potential in human spermatozoa using flow cytometry
Measurement intracellular calcium concentration plasma membrane potential human spermatozoa
2008/12/25
We report 2 novel approaches using flow cytometry to measure intracellular calcium concentration and plasma membrane potential in human spermatozoa. Both approaches have the potential to measure dif...
Measurement of protein C inhibitor in seminal plasma is useful for detecting agenesis of seminal vesicles or the vas deferens
Measurement protein C inhibitor seminal plasma detecting agenesis seminal vesicles vas deferens
2008/12/25
Protein C inhibitor (PCI), a plasma serine protease inhibitor of activated protein C, is present at high concentrations in the seminal plasma of normal subjects and is decreased in some infertile ...
Changes in Testosterone and Dihydrotestosterone Levels in Male Rat Accessory Sex Organs, Serum, and Seminal Fluid After Castration：Establishment of a New Highly Sensitive Simultaneous Androgen Measurement Method
LC-MS/MS hormone prostate seminal vesicles
2008/12/23
It is known that abnormal androgen dynamics in the tissues is a cause of androgen-dependent disorders. Investigation of tissue androgen levels could provide a clue to the elucidation of disorders....
Strength Measurement of the Sertoli-Spermatid Junctional Complex
Ectoplasmic specialization testis micropipette adherens junction
2008/12/23
The Sertoli cell ectoplasmic specialization (ES) is a specialized domain of the calcium-dependent Sertoli cell-spermatid junctional complex. Not only is it associated with the mechanical adhesion of...
Semen Analysis：Setting Standards for the Measurement of Sperm Numbers
Semen Analysis Standards Measurement Sperm Numbers
2008/12/22
Recently several authors have highlighted the need for rigorous quality control as a basis for high-standard quality assurance in the andrology laboratory (Björndahl et al, 2004; Keel, 2004). One...
Enhanced Chemiluminescence Assay vs Colorimetric Assay for Measurement of the Total Antioxidant Capacity of Human Seminal Plasma
Luminometer semen spectrophotometer Trolox reactive oxygen species
2008/12/19
Although the enhanced chemiluminescence assay is commonly used to measure the nonenzymatic total antioxidant capacity (TAC) of the human seminal plasma, it is cumbersome, expensive, and time-consu...
Measurement of Volume Changes in Mouse Spermatozoa Using an Electronic Sizing Analyzer and a Flow Cytometer: Validation and Application to an Infertile Mouse Model
Coulter counter c-ros knockout mice hypo-osmotic swelling regulatory volume decrease
2008/12/5
The importance of sperm volume has recently been highlighted in a knockout mouse model in which infertility was caused by defects in volume reguiation, which led to sperm transport failure in the fe...