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High-level Expression and Purification of Active Human FGF-2 in Escherichia coli by Codon and Culture Condition Optimization
Fibroblast Growth Factor 2 Codon Protein E. coli
2016/1/26
Background: Basic fibroblast growth factor (bFGF) is a member of a highly conserved superfamily of proteins that are involved in cell proliferation, differentiation, and migration.
Objectives: The ob...
Penn Researchers Discover Why E.coli Move Faster in Syrup-like Fluids Than in Water
Penn Researchers E.coli Syrup
2015/12/7
Swimming in a pool of syrup would be difficult for most people, but for bacteria like E. coli, it’s easier than swimming in water. Scientists have known for decades that these cells move faster a...
Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR
E.coli O157:H7 Stx-1 gene Detection FLASH-PCR
2015/9/28
Background: Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome i...
Genotypic Analysis of E. coli Strains Isolated from Patients with Cystitis and Pyelonephritis
Uropathogenic Escherichia coli Pyelonephritis Cystitis Electrophoresis Genetic patterns
2015/9/25
Background: Urinary tract infection is the most common health problem affecting millions of people each year, mainly caused by a large genetically heterogeneous group of Escherichia coli called uropat...
Association of Virulent Genes hly, sfa, cnf-1 and pap with Antibiotic Sensitivity in Escherichia coli Strains Isolated from Children with Community-Acquired UTI
E coli UTI pap hly sfa cnf-1 Drug resistance
2015/9/22
Background: Recent studies have suggested that the decrease in the pathogenicity of E. coli is due to acquisition of resistance to some antibiotics. This study was performed to investigate four virule...
THE FIRST REPORT ON THE ISOLATION OF ENTEROTOXIGENIC ESCHERICHIA COLI AS A CAUSE OF INFANTILE DIARRHEA IN IRAN
Enterotoxigenic Infantile diarrhea Enteropathogenic E.coli
2009/12/28
The role of enterotoxigenic E.coli as a causative agent in diarrheal disease was studied among 100 cases of infant and children 0-2 years of age. Routine bacteriological methods were used for identifi...
TRANSMISSIBLE DRUG RESISTANCE AMONG SALMONELLA TYPHIMURIUM AND ENTEROPATHOGENIC E.COLI IN IRAN
S.typhimurium chloromycetin ampicillin
2009/12/28
The antimicrobial susceptibility of 118 S.typhimurium and 84 enteropathogenic E. coli isolated from human gastero-enteritis cases were examined. More than 25 different resistant patterns could be iden...
Antibiotic Resistance to Third Generation Cephalosporins Due to CTX-M-Type Extended-Spectrum β-Lactamases in Clinical Isolates of Escherichia coli
Escherichia coli CTX-M-β-lactamase ESBL Iran
2009/12/18
Background: Organisms producing CTX-M-β-lactamase are emerging around the world as a source of resistance to oxyiminocephalosporins such as cefotaxime. However, the laboratory detection of these strai...
Determination of antimicrobial resistance patterns and extended spectrum β lactamases in clinical isolates of E. coli Jabalameli F
E.coli extended-spectrum betalactamase antimicrobial resistance
2009/12/9
Comparing Intensity Elicited Maximum Reflex Amplitude Between Noise Induced Hearing Loss & Acoustic Trauma at 1kHZ, Contralaterally, and Investigate Relationship Between Amplitude and Hearing Impairme...
Characterization of Catalytic Phenotype of b-Galactosidase From LacI Mutant,E.Coli CSH-36,as a Tool For The Management of Lactose Intolerance
lactose intolerance b-Galactosidase E.coli purification kinetics
2009/6/30
Objectives: Lactose intolerance is a common public health problem with 5-100% prevalance among different populations of the world. Use of b-galactosidase to reduce the lactose content in food or consu...
Colicin Production and Colicin Typing of Uropathogenic Escherichia coli
Escherichia coli colicin typing urinary tract infections
2009/6/29
Colicins are bactericidal macromolecules in protein structures and they are produced by some strains of Escherichia coli and Shigella, which are members of the Enterobacteriaceae. They have a bacteric...
Carriage of Mobilizable Plasmid-Mediated b-Lactamase Gene in Ampicillin-Resistant Escherichia coli Strains with Origin of Normal Fecal Flora
Fecal flora ampicillin-resistant Escherichia coli mobilizable b-lactamase gene
2009/6/19
Aim: The aim of this study was to investigate the carriage of b-lactamase genes in ampicillin-resistant (Ampr) Escherichia coli (E. coli) isolates from human normal fecal flora. Methods: Ten Ampr E. c...
TNF 家族的新成员TRAIL cDNA 的克隆及其在E. coli 中的表达
Trail 基因克隆 原核表达
2008/10/24
目的:克隆Trail 基因cDNA 全长,构建Trail 的原核表达载体,从而建立其原核表达体系。方法:通过抽提外周血淋巴细胞总RNA 进行RT - PCR 克隆Trail cDNA ,构建Trail 的原核表达载体,用限制性酶切和DNA 测序进行鉴定,以温度进行诱导表达,通过SDS - page 分析表达产物。结果: (1) 克隆了Trail 基因cDNA 全长,DNA 测序结果与报道的完全一致...
指状青霉提取物诱发E.coli ND－160及K12 infA基因突变
指状青霉 大肠杆菌 基因突变
2008/8/25
背景与目的：研究指状青霉(Penicillium digitatum)提取物对大肠杆菌菌株的致突变性。材料与方法：采用E.coli ND－160菌株回复突变试验、K12 infA基因突变试验及其突变序列分析。结果：指状青霉提取物：①可明显地诱导ND－160菌株回复突变；②对K12菌株可诱发其infA基因DNA序列中5个碱基位点突变，且其中1个位点的突变还可导致编码相应氨基酸的改变(Lys→Val)...