搜索结果: 1-12 共查到“家畜繁殖学 PCR”相关记录12条 . 查询时间(0.156 秒)
本研究对5种常用“持家基因”作为鸡肌肉组织发育早期基因和蛋白检测内参基因的有效性进行了评价，为鸡肌肉发育相关分子检测中内参基因的选择提供科学依据。应用实时荧光定量PCR（RT-qPCR）和Western blot技术，分别检测了鸡12胚龄、17胚龄、1日龄和14日龄胸肌中β肌动蛋白（ACTB）、β微管蛋白（TUBB）、TATA结合蛋白（TBP）、甘油醛-3-磷酸脱氢酶（GAPDH）和热休克蛋白70...
本研究旨在建立多重Real-time PCR方法检测猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒2型（PCV2）、猪伪狂犬病毒（PRV）和猪细小病毒(PPV)。根据GenBank数据库中PRRSV、PCV2、PRV、和PPV的核苷酸序列设计特异性引物和探针，通过优化反应条件，检测系列稀释的纯化阳性质粒，建立检测PRRSV、PCV2、PRV和PPV的单项和多重Real-time PCR方法。灵敏...
一例猪繁殖与呼吸综合征的RT-PCR诊断与防制
猪繁殖与呼吸综合征病毒 细胞培养 Marc-145 RT-PCR 诊断 防制
2009/11/5
2008年3月，新乡市某猪场发生了以一种经产母猪和初产母猪流产、死产、产木乃伊胎、死胎及弱仔为特征的疾病。采集流产胎儿和死胎的肺、肝、脾脏等病料，制悬液，取上清，进行细胞培养，观察细胞病变。另根据猪繁殖与呼吸综合征病毒美洲株VR-2332 ORF7基因序列设计一对特异引物，提取流产胎儿的肺、肝等组织中PRRSV的RNA，经RT-PCR扩增，检测猪繁殖与呼吸综合征病毒为阳性，结合病史和临床症状，诊断...
Identification of Spermatogenic Cells Expressing Protamine mRNA in Japanese Quail by RT-PCR
micropipetting protamine mRNA quail RT-PCR spermatogenic cells
2009/7/31
This study was conducted by reverse transcription-polymerase chain reaction (RT-PCR) analysis to determine when the protamine mRNA is expressed in testicular germ cells during spermatogenesis in quail...
中国美利奴羊MHC-DRB1基因PCR-RFLP多态性分析
中国美利奴羊 MHC-DRB1 PCR-RFLP
2008/3/29
应用巢式PCRRFLP方法，对211只中国美利奴（新疆军垦型）羊的MHCDRB1外显子2的遗传多态性进行检测,并对基因型频率和等位基因频率进行了统计，结果表明，中国美利奴（新疆军垦型）羊的MHC-DRB1基因外显子2在SacⅠ、Hin1Ⅰ和HaeⅢ的酶切位点存在多态性，这些酶切位点分别受2、2和6个等位基因控制，由于MHC-DRB1为多碱基突变的基因位点，综合3种限制性内切酶的酶切结果，在中国...
Genetic Similarity Among the Three Egyptian Water Buffalo Flocks Using RAPD-PCR and PCR-RFLP Techniques
water buffalo RAPD-PCR PCR-RFLP phylogeny genetic similarity
2010/11/10
Genetic similarity and polymorphisms among the three Egyptian water buffalo flocks (Beheiry,
Minoufy and Saidy) were studied using both random amplified polymorphic DNA (RAPD) and restriction fragmen...
Sexing Goat Embryos by PCR Amplification of X- and Y- chromosome Specific Sequence of the Amelogenin Gene
Goat Embryos Amelogenin Gene Sexing PCR
2016/4/26
The objective of this study was to develop a simplified, efficient, and accurate protocol for sexing goat embryos. Based on the amelogenin gene located on the conservation region of X- and Y- chromoso...
中国美利奴羊MHC-DRB1基因PCR-RFLP多态性分析
中国美利奴羊 MHC-DRB1 PCR-RFLP
2013/12/26
应用巢式PCRRFLP方法，对211只中国美利奴（新疆军垦型）羊的MHCDRB1外显子2的遗传多态性进行检测,并对基因型频率和等位基因频率进行了统计，结果表明，中国美利奴（新疆军垦型）羊的MHC-DRB1基因外显子2在SacⅠ、Hin1Ⅰ和HaeⅢ的酶切位点存在多态性，这些酶切位点分别受2、2和6个等位基因控制，由于MHC-DRB1为多碱基突变的基因位点，综合3种限制性内切酶的酶切结果，在中国...
2个绵羊二核苷酸微卫星BM143和BMS2508的PCR扩增产物在非变性（中性）聚丙烯酰胺凝胶电泳（nPAGE）中形成滞后于主带的影子带。影子带比主带小2 bp。实际上主带由两部分组成：表明微卫星正确大小的PCR特异扩增的高浓度条带（特异扩增带）和比特异扩增带小2 bp的PCR非特异扩增的低浓度条带（非特异扩增带）。本实验初步证明影子带是微卫星的一条特异扩增带和一条非特异扩增带错配形成的异源双链。...
Assessment of Bandsharing Values in RAPD-PCR Analysis of Dwarf Cattle of Kerala
Random Amplified Polymorphic DNA Marker Bandsharing Value Dwarf Cattle
2016/4/19
Random amplified polymorphic DNA (RAPD-PCR) analysis of 56 animals of four different genetic groups of dwarf cattle in Kerala was done as a single step analysis. Bandsharing (BS) values were calculate...
Association between PCR-RFLP of Melatonin Receptor 1a Gene and High Prolificacy in Small Tail Han Sheep
Sheep Prolificacy Reproductive Seasonality Melatonin Receptor 1a Gene PCR-RFLP
2016/4/14
Melatonin regulates circadian rhythms and reproduction changes in seasonally reproductive mammals through binding to high-affinity, G-protein-coupled receptors. Small Tail Han sheep that has significa...