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Tissue-specific mRNA expression profiles of GDF9,BMP15,and BMPR1B genes in prolific and non-prolific goat breeds
goat prolificacy ovary RT-PCR gene expression
2015/10/29
The tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in goats were investigated. The mRNA expression of the genes was detected in prolific Jining Grey goats and non-prolific L...
GDF9下调转基因小鼠模型对繁殖与生长的影响
RNA干涉 繁殖 转基因小鼠
2015/7/28
本研究旨在对转GDF9 shRNA基因小鼠进行扩群繁殖，统计分析转基因小鼠的产仔数及增重，检测GDF9 shRNA、GDF9表达水平以及血清中FSH和GH的水平，以观察GDF9的下调对转基因小鼠繁殖性能及生长发育的影响。通过RT-PCR方法，分别检测了GDF9 shRNA在体内的表达，以及下丘脑、垂体和卵巢中GDF9 mRNA表达水平；同时用ELISA检测了血液中FSH和GH水平。结果表明，扩群后...
Association of Polymorphisms in Fecundity Genes of GDF9, BMP15 and BMP15-1B with Litter Size in Iranian Baluchi Sheep
BMP15 BMP15-1B GDF9 Litter Size Baluchi Sheep
2016/5/6
The incidence of mutation in three loci of GDF9, BMP15 and BMP15-1B and their effects on litter sizes was evaluated in Baluchi sheep. Wild-type alleles were detected for BMP15 and BMP15-1B loci and al...
牛GDF9和BMP15基因遗传变异与双胎性状的关系研究
牛 生长分化因子9基因 骨形态发生蛋白15基因 双胎 翻译起始位点 RNA二级结构
2013/12/27
以生长分化因子9(Growth differentiation factor 9, GDF9)基因和骨形态发生蛋白15 (Bone morphogenetic protein 15, BMP15)基因作为牛双胎性状的候选基因，研究了它们在鲁西牛、秦川牛、南阳牛和中国荷斯坦牛4个品种中的遗传变异，并在鲁西牛群体中研究了其多态位点与双胎性状的关系。结果表明：在鲁西牛中GDF9基因的3′UTR 发现缺失...