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Analysis of Arabidopsis seedling responses to daytime temperature regimes identifies an mRNA hairpin as a novel thermosensor in plants.
Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis
Vector Target mimic miRNA, Cloning Plant Downregulation
2024/1/19
Small RNAs are sequence-dependent negative regulators of gene expression involved in many relevant plant processes such as development, genome stability, or stress response. Functional characterizatio...
Live single-cell transcriptional dynamics via RNA labelling during the phosphate response in plants
RNA single-cell transcriptional dynamics phosphate response plant development
2023/12/1
Plants are constantly adapting to ambient fluctuations through spatial and temporal transcriptional responses. Here, we implemented the latest-generation RNA imaging system and combined it with microf...
A Picea wilsonii NAC transcription factor, PwNAC1, interacts with RNA-binding protein PwRBP1 and synergistically enhances drought and salt tolerance of transgenic Arabidopsis
Picea wilsonii Transcription factor PwNAC1 PwRBP1 Abiotic stress ABA signaling
2023/11/16
NAC transcription factors play crucial roles in plant resistance to a variety of adverse circumstances. Here, we characterized a NAC transcription factor PwNAC1 from Picea wilsonii and subcellular loc...
Two environmental signal-driven RNA metabolic processes: alternative splicing and translation
alternative splicing environmental signal receptor kinase RNA metabolism
2023/11/16
Plants live in fixed locations and have evolved adaptation mechanisms that integrate multiple responses to various environmental signals. Among the different components of these response pathways, rec...
银杏叶片RNA提取方法的研究
银杏叶片 RNA提取方法
2011/9/1
为了获得一种银杏（Ginkgo biloba L.）叶片总RNA提取的理想方法，为后续的分子生物学研究打下基础，笔者以不同生长期的叶片为材料，利用生物分光光度计和凝胶电泳法比较了改良CTAB法、乙二醇丁醚法以及改良Trizol法提取的银杏总RNA的产率和纯度。结果表明：改良Trizol法所提取RNA的OD260/OD280比值介于1.8~2.0之间，并且OD260/OD230在1.9~2.1之间，...