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【目的】研究不同温度对果梅花柱S-RNase降解花粉管RNA的影响,为揭示果梅自交不亲和性机制提供依据。【方法】在液体培养基中添加从果梅‘月世界’花柱中纯化的S-RNase,并分别于10℃、25℃和35℃条件下培养自花(‘月世界’)、异花(‘莺宿’)花粉,测定不同培养时间后的花粉萌发率和花粉管生长长度;离体条件下进行果梅花柱S-RNase降解花粉管RNA反应后,用琼脂糖凝胶电泳检测不同温度条件下自...
Development of the Sf-RNase Gene-Specific PCR Primer Set for Japanese Apricot (Prunus mume Sieb. et Zucc.)
PCR Japanese Apricot S-RNase
2009/7/13
Self-compatible cultivars of Japanese apricot (Prunus mume Sieb. et Zucc.) have a horticultural advantage over self-incompatible ones because no cross-pollinizer tree is required. Self-incompatibility...