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Preparation of Microprotoplasts for Partial Genome Transfer via Microprotoplast Fusion in Liliaceous Ornamental Plants
cell suspension culture Hemerocallis hybrida Lilium longiflorum micronucleation microsporocyte
2009/9/10
We aimed to produce intergeneric hybrid plants with only one or a few alien chromosomes via microprotoplast fusion for genetic improvement and chromosome studies in Liliaceous ornamental plants. In or...
Directional transfer of the genic multiple allele inherited male sterile line in Chinese cabbage
Chinese cabbage genetic male sterile line transfer
2010/9/10
A “directional transfer program” was designed and carried out according to the genotype on the male sterility locus of the target line, in order to improve the utilization of the multiple-allele inher...
抗菌肽D基因导入番茄及转基因植株的鉴定Transfer of Antibacterial Peptide D Gene into Tomato and Identification of Transgenic Plants
抗菌肽D基因 花粉通道法 番茄 青枯病
2007/12/7
摘要用花粉管通道法将柞蚕抗菌肽D基因导入番茄，获得了转基因植株。应用PCR技术、DNA斑点杂交以及Southern印迹分析表明，抗菌肽D基因已整合到番茄基因组；而且，位于目的基因下游的胭脂碱合成酶基因有表达。青枯假单孢菌活体接种结合高发病大田种植试验结果显示，部分转基因植株的子一代具有较强的抗青枯病能力。
Abstract:By using pollen-tube pathway,antibac...