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Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis
CRISPR-Cas12a Temperature Genome editing Rice Arabidopsis Maize
2023/8/9
CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct features that have expanded genome editing capabilities. Cas12a-mediated genome editing is temperature sensitive in plants, bu...
Predicting Intron Sites by Aligning Cotton ESTs with Arabidopsis Genomic DNA
Intron Aligning ESTs Arabidopsis Genomic DNA
2008/12/9
Among the transcribed regions of a gene, introns are the most polymorphic; therefore, they are ideally suited for developing molecular markers. The identification of intron regions, however, is not a ...
Isolation and functional characterization of two rapeseed genes orthologous to Arabidopsis thaliana Phenylalanine Ammonia-lyase 2
Arabidopsis thaliana Brassica napus L ortholog phenylalanine ammonia-lyase (PAL) yellow seed
2010/1/11
Two rapeseed (Brassica napus L.) phenylalanine ammonia-lyase 2 genes (BnPAL2-1 and BnPAL2-2) were isolated. The full-length cDNAs of BnPAL2-1 and BnPAL2-2 are 2422 bp and 2421 bp, with corresponding g...