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The role of nitric oxide synthase isoforms in aged porcine oocytes
nitric oxide L-NAME aminoguanidine oocyte ageing pig
2015/6/24
In the sphere of reproductive biotechnologies, the demand for sufficient numbers of high-quality oocytes is still increasing. In some cases, this obstacle is overcome by in vitro prolonged cultivation...
Effects of inhibiting nitric oxide synthase on cumulus expansion and nuclear maturation of sheep oocytes
sheep oocyte maturation nitric oxide L-NAME
2015/6/9
Nitric oxide (NO) is a biological signaling molecule that plays a crucial role in oocyte maturation of mammalians. It is generated by the nitric oxide synthase (NOS) enzyme from l-arginine. Although t...
Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor
activation nitric oxide oocyte
2015/6/8
Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor SNAP (2mM). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. ...
Production of Boer goat (Capra hircus) by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes
goat fibroblast clone
2015/6/8
The present study reports a birth of goat clone produced by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes. The donors of somatic cells were fibrob...
Porcine oocytes matured in vitro develop in various ways if they are further cultivated. In our studies these oocytes were cultivated for 1 to 5 days (in vitro aging). During the 1st day of aging, mos...
Effects of genistein and genistin on in vitro maturation of pig oocytes
phytoestrogen isoflavone oocyte maturation pig estrogenic effect tyrosine protein kinase
2015/6/5
The objective of the study was to verify the hypothesis that GEN (genistein – phytoestrogen and an inhibitor of tyrosine protein kinase – TPK) effects on pig oocyte maturation and cumular cell expansi...
Parthenogenetic development of rabbit oocytes after electrical stimulation
rabbit oocytes electric pulses in vitro culture
2015/6/2
The aim of this study was to determine the effect of electric pulses on the structural and functional condition of rabbit oocytes. The New Zealand White female rabbits at 3–5 months of age and at 3–4 ...
Causes and consequences of maternal age-related aneuploidy in oocytes: a review
aneuploidy oocyte maternal age meiosis spindle assembly checkpoint
2015/4/17
Although a positive correlation between aneuploidy and maternal age was first reported almost a century ago, the underlying mechanisms remain mostly unknown. Different hypotheses regarding age-related...
Expression of cyclin-dependent kinase inhibitors (CDKN1, CDKN5) in developmentally competent and incompetent porcine oocytes
Cdkn1 Cdkn5 oocyte developmental competence
2015/4/16
Although several reports have been published regarding the cyclin-dependent kinases (Cdks) in developmentally competent mammalian oocytes, still little is known about their expression pattern in devel...
Maternal nutrition affects the composition of follicular fluid and transcript content in gilt oocytes
fatty acids BCB test pig EEF1A1 ATP5A1
2015/4/15
Metabolomics focused on reproduction have been the subject of special interest in the past decade. Metabolite profiling provides detailed information on the status of follicular fluid and follicular c...
The morphology of porcine oocytes is associated with zona pellucida glycoprotein 3 and integrin beta 2 protein levels--
pig oocyte morphology
2015/4/8
The morphology and quality of oocytes is set during oo- and folliculo-genesis, and is completed during final maturation. Early embryonic development is associated with the morphology of the cumulus-oo...
In vitro maturation and degeneration of domestic cat oocytes collected from ovaries stored at various temperatures
IVM feline COC apoptosis necrosis
2015/4/1
Cat oocytes have the unique ability to mature in vitro after temporary storage at 4°C which can provide opportunities to rescue oocytes from the ovaries of endangered felids after sudden death or medi...
Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro
gilts follicular development oocytes morphology meiotic competence
2015/3/17
The objective of the study was to characterize the effect of the oestrous cycle stage on the yield,
morphology and meiotic competence of porcine oocytes. A total of 46 cycling gilts, at 8.5–9 months...
Collection of oocytes from donors in the growth phase of follicular development can enhance the production of bovine embryos for cryopreservation
follicular development bovine oocyte in vitro embryos cryopreservation
2015/3/5
The present study was designed to compare the efficiency of bovine embryo production for cryopreservation between oocytes collected from donors in the growth phase of follicular development (GPFD) and...
Effects of superstimulation with fsh on follicular population and recovery rate of oocytes in the growing phase of the first and second follicular wave
ovum pick-up superstimulation cattle
2015/1/26
Effectiveness of in vitro production of embryos (IVP) is limited among other factors by the recovery rate of oocytes. Gonadotropin superstimulation can improve the recovery rate of oocytes. The effect...