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Efficient Gene Transfer into Early Chicken Embryos by Electroporation of Stage X Blastoderms in Vivo, Applying Electric Pulses Vertically to the Blastoderm Layer
blastoderm chick embryo electroporation embryo culture GFP gene
2009/7/31
In order to introduce exogenous DNA into early chicken embryos by transfecting the stage X (EG&K) blastoderm, in vivo electroporation was applied to it. A pair of novel electrodes was devised for appl...
Effects of Soft X-Ray Irradiation to Stage X Blastoderm on Restriction of Proliferation of Primordial Germ Cells in Early Chicken Embryos
chick embryo primordial germ cell soft X-ray stage X blastoderm
2009/7/30
It is necessary to deplete the endogenous primordial germ cells (PGCs) from recipient embryos when germline chimaeric chickens are produced by the transfer of PGCs. In this study, effects of soft X-ra...
Effects of Busulfan Sustained-release Emulsion on Depletion and Repopulation of Primordial Germ Cells in Early Chicken Embryos
busulfan sustained-release emulsion early chicken embryos germline chimeras primordial germ cells
2009/7/29
The current study aimed to determine the optimal time of busulfan (1,4-butanediol dimethanesulfonate) administration for the preparation of suitable recipient embryos to generate germline chimeras in ...