搜索结果: 1-15 共查到“农学 T-RFLP”相关记录108条 . 查询时间(0.093 秒)
A relationship between the PCR-RFLP polymorphism in porcine MYOG, MYOD1 and MYF5 genes and microstructural characteristics of m. longissimus lumborum in Pietrain × (Polish Large White × Polish Landrace) crosses
MyoD genes muscle fibres pig
2015/6/5
Muscle fibre formation takes place during embryonic development and is regulated by the MyoD gene family, which consists of four genes, MYOD1, myogenin, MYF5 and MRF4. A relationship was studied betwe...
A DdeI PCR-RFLP detecting a novel missense mutation of the POU1F1 gene showed no effects on growth traits in cattle
bovine POU1F1 gene PCR-RFLP growth traits
2015/6/5
In this study, a novel missense (NM_174579:c.1201C>T) mutation in exon 6 at the bovine POU1F1 locus is reported, which results in p.S284F, namely, Ser (TCT) > Phe (TTT) at position 284 of the mature p...
Human-to-human and human-to-dog Mycobacterium tuberculosis transmission studied by IS6110 RFLP analysis:a case report
zoonosis human tuberculosis pet animal
2015/4/15
This study reports on the transmission of Mycobacterium tuberculosis of the same IS6110 RFLP type between two acquaintances with open pulmonary tuberculosis and a five-year-old Doberman bitch. No clin...
Genotyping of Mycobacterium avium subsp. avium isolates from domestic animals in Slovenia by IS901 RFLP
genotyping mycobacteriosis avian tuberculosis
2015/4/2
ABSTRACT: Apart from birds, Mycobacterium avium subsp. avium (MAA) is often isolated from granulomatous lesions in pigs and occasionally from cattle and other animals. The objectives of this study wer...
Identification of Pork Contamination in Meatballs of Indonesia Local Market Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis
Pork Contamination Detection Meatballs Polymerase Chain Reaction
2016/5/17
This research applied and evaluated a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using cytochrome b gene to detect pork contamination in meatballs from local markets...
本研究应用PCR-RFLP(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism)和PCR-DGGE(Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis)技术，对工厂化养殖的凡纳滨对虾肠道菌群进行多样性分析。RFLP结果显示，8月样品中不同...
2012年初，山东菏泽某羊场的羊群发病，从发病羊器官分离到2株病原细菌。对病原细菌进行鉴定，并对其与已知同种异源菌株相似性差异进行分析。从患病山羊内脏器官分离细菌，经形态特征、培养特性、生化试验、血清学试验及致病性试验进行鉴定；再通过设计通用引物扩增16S-23S rRNA ISR (intergenic spacer region)序列，将PCR产物经HinfⅠ单酶切获得3条可视条带，同时对扩增...
本研究旨在建立猪瘟病毒野毒株和兔化弱毒疫苗株RTPCRRFLP鉴别检测方法。根据Shimen株设计1对特异性引物，建立猪瘟病毒 RTPCRRFLP检测方法；对20份疑似猪瘟临床样品进行检测，并对检出的山东8株流行野毒株和2株疫苗株PCR产物进行克隆与序列分析，验证上述方法。结果RTPCR扩增片段为825 bp，产物经RFLP分析，野毒株的PCR产物能被ApaⅠ酶切为322 bp和503 ...
本研究旨在建立猪瘟病毒野毒株和兔化弱毒疫苗株RT-PCR-RFLP鉴别检测方法。根据Shimen株设计1对特异性引物，建立猪瘟病毒 RT-PCR-RFLP检测方法；对20份疑似猪瘟临床样品进行检测，并对检出的山东8株流行野毒株和2株疫苗株PCR产物进行克隆与序列分析，验证上述方法。结果RT-PCR扩增片段为825 bp，产物经RFLP分析，野毒株的PCR产物能被ApaⅠ酶切为322 bp和503 ...
T-RFLP分析技术在肉鸡肠道微生物研究中的应用
T-RFLP分析技术 肉鸡 肠道微生物
2013/11/20
本文阐述了末端限制性片段长度多态性(T-RFLP)分析技术的基本原理和技术路线，讨论了应用T-RFLP分析技术相对于应用传统方法研究肉鸡肠道微生物的优势，总结了应用T-RFLP分析技术研究肉鸡肠道微生物的发展历程，分析了应用T-RFLP分析技术研究肉鸡肠道微生物存在的问题及解决方法，并对其应用前景进行了展望。
以17株标准菌株为参照，采用限制性片段长度多态性（RFLP）分析和16S rDNA序列分析相结合的技术，对分离自蒙古国5个地区传统乳制品中的55株乳酸菌进行了快速鉴定。结果表明,通过限制性内切酶AluⅠ、HaeⅢ、BsmaⅠ、TspRⅠ和HinfⅠ的指纹图谱分析，将49株乳杆菌和2株片球菌准确鉴定到种，其他4株肠球菌鉴定到属。采用16S rDNA-RFLP方法鉴定乳酸菌具有准确性，可靠性和高效性。...
琅琊鸡Mx基因3’端部分序列PCRRFLP与序列分析
琅琊鸡 Mx基因 PCRRFLP 序列分析
2010/1/8
［目的］为琅琊鸡分子标记辅助选择、抗病育种提供依据。［方法］利用PCRRFLP技术对琅琊鸡Mx基因3’端片段的Hae Ⅲ的酶切多态性进行分析。［结果］琅琊鸡群体中存在Mx基因Hae Ⅲ酶切位点的多态性，且均有A、B 2个等位基因，基因频率分别为0.562和0.438。Mx基因Hae Ⅲ酶切位点的基因型分布在琅琊鸡群体中极显著偏离HardyWeinberg平衡状态(...
水稻是宁夏地区主要粮食作物, 水稻种植也具有维持生态系统平衡, 防止土地荒漠化等重要的生态功能。而稻田土壤细菌是维持土壤生态功能的基础。但长期以来缺乏对干旱地区稻田土壤细菌多样性的认识。本研究采用非培养技术提取稻田土壤样品总DNA, 构建其16S rDNA克隆文库, 用PCR-RFLP分析进一步测序后聚类分析细菌群落的多样性。从稻田土样中分离获得了大于23 kb的DNA片段。PCR-RFLP共得到...
PCRRFLP技术检测嘉兴凤桥种猪氟烷基因
凤桥 猪 氟烷基因 PCR RFLP
2009/9/16
［目的］ 为凤桥镇通过合理利用氟烷基因提高猪肉品质奠定理论基础。［方法］ 从嘉兴县凤桥镇的规模化养猪场随机抽取种猪血样，通过PCRRFLP技术进行氟烷基因检测。［结果］ HhaⅠ内切酶的识别位点为5′GCG↓C3′。PCR扩增产物是含RYR1基因第1 843位点长度为659 bp的片段。如果猪的基因型为NN，由于未发生胞嘧啶C到胸腺嘧啶T的突变，可以看到2条带。如果猪的基因型为nn，由...
Discrimination among Japanese Species of the Orius Flower Bugs (Heteroptera: Anthocoridae) Based on PCR-RFLP of the Nuclear and Mitochondrial DNAs
natural enemy predatory bugs species identification internal transcribed spacer 1 cytochrome oxidase subunit I
2009/9/9
A PCR-RFLP-based method of species identification was considered for 5 Japanese species of Orius Wolff flower bugs, Orius strigicollis (Poppius), O. minutus (Linnaeus), O. sauteri (Poppius), O. nagaii...