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Covalent Self-labeling of Tagged Proteins with Chemical Fluorescent Dyes in BY-2 Cells and Arabidopsis Seedlings
BY-2 cells Arabidopsis SNAP-tag live cell imaging microtubules endocytosis PIN2 auxin transporter
2023/12/21
Synthetic chemical fluorescent dyes promise to be useful for many applications in biology. Covalent, targeted labeling, such as with a SNAP-tag, uses synthetic dyes to label specific proteins in vivo ...
Protein kinase sensors: an overview of new designs for visualizing kinase dynamics in single plant cells
plant cells protein kinase sensors kinase dynamics signaling networks SnRK2
2023/12/1
Protein kinase dynamics play key roles in regulation of cell differentiation, growth, development and in diverse cell signaling networks. Protein kinase sensors enable visualization of protein kinase ...
Cross-talk between clathrin-dependent post-Golgi trafficking and clathrin-mediated endocytosis in Arabidopsis root cells
Adaptor protein Arabidopsis TPLATE clathrin endocytosis exocytosis
2023/12/1
Coupling of post-Golgi and endocytic membrane transport ensures that the flow of materials to/from the plasma membrane (PM) is properly balanced. The mechanisms underlying the coordinated trafficking ...
Single-cell transcriptomics sheds light on the identity and metabolism of developing leaf cells
Single-cell transcriptomics photosynthetic Arabidopsis thaliana high-throughput transcriptome metabolic stress-related
2023/11/30
As the main photosynthetic instruments of vascular plants, leaves are crucial and complex plant organs. A strict organization of leaf mesophyll and epidermal cell layers orchestrates photosynthesis an...
学术报告:IIIandMe: An Algorithm for Chromosome-scale Haplotype Determination Using Genome-wide Variants of Three Haploid Reproductive Cells
Algorithm Chromosome-scale Haplotype Determination
2023/7/20
贾震宇教授,本科就读于武汉大学生命科学学院生物化学专业。2006年于加州大学河滨分校同时取得统计学硕士和遗传-基因组-生物信息联合学科博士。先后就职于加州大学尔湾分校病理系、阿克伦大学统计系和俄亥俄西北医科大学医学系,从事包括前列腺癌在内的多种疾病的研究以及在生物统计和生物信息学科的教学工作。现受聘为加州大学河滨分校植物科学系数量遗传学教授。贾老师目前的研究工作包括基因组选择方法在作物育种和癌症预...
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中国科学院武汉植物园应邀在Cells上发表研究综述(图)
病原菌胁迫 生理生化 营养物质
2022/10/26
果实成熟,是指经过一系列生理生化变化而达到最佳食用状态的过程,是果实品质形成的必要前提。然而,随着果实成熟,其对病原菌的抗性也显著降低,导致一系列采后病害的发生,造成巨大的经济损失。传统贮藏保鲜技术通常需要抑制或延缓成熟进程,以牺牲成熟品质为代价,获得较长的货架期,这也导致果实风味和营养物质的严重丧失。因此,如何在有效维持果实成熟品质的同时,增强其对病原菌的抗性是采后生物学研究的重要课题。
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FASE刊“Large Animal Stem Cells”专辑综述文章全面阐述猪多能性干细胞最新研究进展及科学展望(图)
FASE刊 Large Animal Stem Cells 综述文章 猪 多能性干细胞 动物育种应用
2019/3/12
多能干细胞(PSC)因其具有自我更新和多向分化潜能,在体外可以长期培养并能进行基因编辑,其在生命科学与医学领域,以及动物育种应用等领域备受青睐。然而,大家畜,如猪,至今没有获得能够进行生殖嵌合、稳定传代、体外长期培养的多能干细胞系。这也是制约大家畜育种的瓶颈。自第一株小鼠胚胎干细胞报道以来,科学家对猪多能干细胞进了30多年的攻关,虽取得了良好的进展,但仍存在急需解决的科学问题。近日,由中国农业大学...
Retinoic acid attenuates oxidative injury in bovine mammary epithelial cells induced by hydrogen peroxide
vitamin A thioredoxin reductase oxidative injury
2017/12/25
The objective of this study was to explore how retinoic acid (RA) attenuates oxidative injury induced by hydrogen peroxide (H2O2) in bovine mammary epithelial cells (BMEC). Subconfluence BMEC were ran...
Retinoic Acid Attenuates Oxidative Injury in Bovine Mammary Epithelial Cells Induced by Hydrogen Peroxide
vitamin A thioredoxin reductase oxidative injury arachidonic acid p38 mitogen-activated protein kinase
2018/3/5
Jin L., Yan S., Shi B., Shi H., Guo X., Li J. (2017): Retinoic acid attenuates oxidative injury in bovine
mammary epithelial cells induced by hydrogen peroxide. Czech J. Anim. Sci., 62, 539–548.
The...
Lignosulfonic acid promotes hypertrophy in 3T3-L1 cells without increasing lipid content and increases their 2-deoxyglucose uptake
Adipogenesis Diabetes Dietary Fiber Glucose Uptake Lignosulfonic Acid
2017/3/7
Adipose tissue plays a key role in the development of obesity and diabetes. We previously reported that lignosulfonic acid suppresses the rise in blood glucose levels through the inhibition of α-gluco...
Effects of retinoic acid on the synthesis of selenoprotein and the antioxidative indices of bovine mammary epithelial cells in vitro
vitamin A antioxidant function dairy cows
2016/5/11
The present study was conducted to examine the effects of retinoic acid (RA) on the synthesis of selenoprotein and the antioxidative indices of bovine mammary epithelial cells (BMEC) in vitro and to e...
Vitamin A affects the expression of antioxidant genes in bovine mammary epithelial cells with oxidative stress induced by diethylene triamine-nitric oxide
nitric oxide antioxidant enzymes inflammatory factors
2016/3/24
The considerable increase in oxygen requirements due to the high metabolic rate of the bovine mammary epithelial cells (BMEC) during lactation results in an augmented production of reactive nitrogen s...
Dynamical Expression of MicroRNA-127-3p in Proliferating and Differentiating C2C12 Cells
MiR-127-3p C2C12 Proliferation Differentiation Goats
2016/12/28
MicroRNAs (miRNAs) are highly conserved, short non-coding RNAs that regulate gene expression at the posttranscriptional level. Although many miRNAs are identified in muscles and muscle cells, their in...
Effects of Extracellular Matrix Protein-derived Signaling on the Maintenance of the Undifferentiated State of Spermatogonial Stem Cells from Porcine Neonatal Testis
Porcine Spermatogonial Stem Cells Undifferentiation, Extracellular Matrix Proteins
2016/11/10
In general, the seminiferous tubule basement membrane (STBM), comprising laminin, collagen IV, perlecan, and entactin, plays an important role in self-renewal and spermatogenesis of spermatogonial ste...
Rapamycin Inhibits Expression of Elongation of Very-long-chain Fatty Acids 1 and Synthesis of Docosahexaenoic Acid in Bovine Mammary Epithelial Cells
Bovine Mammary Epithelial Cells Mammalian Target of Rapamycin Complex
2016/11/10
Mammalian target of rapamycin complex 1 (mTORC1) is a central regulator of cell growth and metabolism and is sufficient to induce specific metabolic processes, including de novo lipid biosynthesis. El...