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Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments
porcine assisted reproductive technology
2015/7/7
While no cleavage was observed in the
CYS group, no significant differences in the cleavage rate were observed between control (21.3%) and TX (10.5%)
groups. In summary, and under our conditions, ...
Developmental Enhancement of Intracytoplasmic Sperm Injection (ICSI)-Generated Quail Embryos by Phospholipase Cζ cRNA
blastoderm embryo development ICSI phospholipase Cζ quail
2009/7/30
The present study was conducted to improve the rate and stage of blastoderm and embryo development of quail oocytes by intracytoplasmic sperm injection (ICSI) with quail phospholipase Cζ (PLCζ) cRNA. ...
不同激活方法对猪ICSI卵早期发育的影响
猪 卵母细胞 ICSI 电激活
2009/7/3
0 .05) ; ICSI 后采用不同激活方法激活卵母细胞, 检测猪ICSI 卵胚胎早期发育情况, 单纯电脉冲激活囊胚率达13 .0 %, 显著高于对照( P <0 .05) 。[ 结论] 单纯电脉冲激活能促进猪ICSI 胚胎的早期发育。
[目的]探讨不同状态奶牛精子卵胞质内单精注射(ICSI)的效果。[方法] 体外成熟培养24 h的奶牛卵母细胞注入不同状态精子,观察其体外发育情况。[结果]结果表明,试验1,研究精子用5 mmol DTT(二硫苏糖醇)预处理与精子不经过预处理的对照组的效果,经DTT处理后,精子解聚率和雄原核率显著高于对照组(30.4%和11.6%,47.8%和27.9%,P<0.05),两组的卵裂率差异不显著(8...
影响山羊胞质内单精子注射(ICSI)效果的因素研究
山羊 胞质内单精子注射(ICSI)效果
2009/6/11
从鲜精与冻精、激活方法以及气相条件3个方面,开展影响山羊卵母细胞质内单精子注射(ICSI)效果的因素研究。结果发现:冻精受精率显著高于鲜精(52.21% VS 26.44%,P<0.05);与离子霉素5 min+6-DMAP 3 h联合激活相比,离子霉素单独处理15 min能相对安全、简捷地激活ICSI后的山羊卵母细胞;低氧(5% O2)气相条件下的囊胚率(19.59%)显著高于高氧(20% O2...