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APC/CCDC20 targets SCFFBL17 to activate replication stress responses in Arabidopsis
APC/C DNA replication stress SCF WEE1 ubiquitination
2023/11/16
DNA replication stress threatens genome stability and affects plant growth and development. How plants resolve replication stress is poorly understood. The protein kinase WEE1-mediated cell cycle arre...
猪miR-135a-5p预测靶基因APC的鉴定
猪 miR-135a-5p APC 靶标
2015/5/28
本研究旨在鉴定猪miR-135a-5p靶向脂肪发育的相关基因。首先,利用生物信息学软件分析发现,结肠腺瘤性息肉病(APC)基因的3′ 非翻译区(3′ UTR)具有两个与猪miR-135a-5p结合的潜在位点。其次,构建APC基因野生型(APC 3′ UTR)和突变型(APC 3′ UTR m1、APC 3′ UTR m2、APC 3′ UTR m3)3′ UTR双荧光素酶载体,并分别将其与miR-...