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Quantification of parvalbumin in commercially important Mediterranean seafood species using real time PCR
protein quantification Real Time PCR seafood allergy
2015/3/30
The parvalbumin allergen gene was quantified in various types of seafood using RT PCR. Freshly harvested specimens from 25 species of finfish, molluscs, and crustacean shellfish, commonly consumed in ...
Accessing The Bioavailability Of Phytochemicals In CACO-2 Cell Model And Developing A Sensitive Method For The Detection And Quantification Of These Compounds
Polymethoxyflavones Transport Permeability
2014/10/22
Numerous studies have found certain unmethylated phytochemicals to possess anti-carcinogenic activity; however, they have been associated with poor oral bioavailability which is a major limiting facto...
Effect of high temperature and pressure on quantification of MON 810 maize
DNA degradation PCR highly processed foods
2014/2/24
Maize MON 810 (Zea mays L.) is the only transgenic cultivar grown in the European Union countries and food products with its content higher than 0.9% must be labelled. Processing such as high temperat...
The Employment of FTIR Spectroscopy and Chemometrics for Classification and Quantification of Mutton Fat in Cod Liver Oil
Spectroscopy and Chemometrics Classification
2016/6/1
In pharmaceutical field, Cod Liver Oil (CLO) is one of the potential sources of long chain omega-3 fatty acids and fat soluble vitamins, especially vitamin A. In the fats and oils industry, CLO has hi...
Reliability of PCR based screening for identification and quantification of GMOs
GMO (Genetically Modified Organism) identification PCR real-time PCR screening RR soya
2014/2/27
Handling with genetically modified organisms (GMOs) is regulated namely in EC. Laboratories often use polymerase chain reaction (PCR) based screening methods to monitor the presence of GM particles in...
Convenient Analysis and Quantification of Diferulic Acids in Foods
diferulic acids ferulic acid cross-link
2009/7/8
A convenient analytical method for diferulic acids (diFAs) in foods was developed. Rice flour (10 g) was stirred in 250 ml of 0.1 N NaOH at room temperature for 50 h. Liberated phenolics were extracte...
Quantification of Light-Induced Glycoalkaloids, α-Solanine and α-Chaconine, in Four Potato Cultivars（Solanum tuberosum L.）Distributed in Japan by LC/MS
solanum tuberosum potato glycoalkaloids
2009/6/18
The major glycoalkaloids, α-solanine and α-chaconine, in potato tubers were determined by LC/ESI-MS. The average recoveries from spiked samples by the method used in this study were more than 94% with...
Quantification of Nobiletin and Tangeretin in Citrus by Micellar Electrokinetic Capillary
nobiletin tangeretin polymethoxylated flavone
2009/5/31
A micellar electrokinetic capillary chromatographic (MEKC) method was developed for the two major polymethoxylated flavones, nobiletin and tangeretin, using a fused-silica open-tubular capillary in a ...
Isolation and Quantification of Triterpenoid Acids from Ganoderma applanatum of Istrian Origin
Ganoderma applanatum triterpenoids ganoderic acids
2009/3/23
Various Ganoderma spp. have been known for their wide range of pharmacologically active compounds. From Ganoderma applanatum of Istrian origin, growing on Populus, triterpenoid acids were isolated and...
Analysis and Quantification of trans-Resveratrol in Wines from Alentejo Region
trans-resveratrol wines HPLC method validation
2009/3/18
A simple procedure for determination of trans-resveratrol in wines from Alentejo region delimited appellation (Portugal) is described and validated. A set of 47 red and 21 white wines was analysed by ...
Comparison of Three RT-PCR Based Methods for Relative Quantification of mRNA
aminopeptidase N APN competitive PCR
2009/3/16
Comparison of three RT-PCR based methods: semi-quantitative, competitive and real-time RT-PCR for relative quantification of mRNA is presented. Aminopeptidase N expressed on human promyeloid HL-60 cel...