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Knock-in of Enhanced Green Fluorescent Protein or/and Human Fibroblast Growth Factor 2 Gene into β-Casein Gene Locus in the Porcine Fibroblasts to Produce Therapeutic Protein
Knock-in Homologous Recombination Therapeutic Proteins Porcine β-casein Gene
2016/5/17
Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjectio...
Recent Progress in Biotechnology-based Gene Manipulating Systems to Produce Knock-In/Out Mouse Models
Transgenic Knock In/Out ROSA26 Tetraploid ES Cells Gene Manipulation
2016/4/27
Gene-manipulated mice were discovered for the first time about a quarter century ago. Since then, numerous sophisticated technologies have been developed and applied to answer key questions about the ...
基因敲除是研究生物体基因功能的有效手段。通过基因敲除建立的鼠疾病模型，在研究基因功能及人类疑难病症致病机制等方面发挥着前所未有的作用。本文对目前已获得的基因敲除鼠疾病模型进行了分类和总结，为相关研究的展开奠定了基础。