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Heat Shock Protein Augmentation of Angelica gigas Nakai Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes
Angelica gigas Nakai Heat Shock Heat Shock Protein 3T3-L1 Adipogenesis
2016/5/19
There is a high association of heat shock on the alteration of energy and lipid metabolism. The alterations associated with thermal stress are composed of gene expression changes and adaptation throug...
Zinc-chelated Vitamin C Stimulates Adipogenesis of 3T3-L1 Cells
Adipogenesis PPARγ C/EBPα GLUT4 3T3-L1
2016/5/12
Adipose tissue development and function play a critical role in the regulation of energy balance, lipid metabolism, and the pathophysiology of metabolic syndromes. Although the effect of zinc ascorbat...
Differential Action of trans-10, cis-12 Conjugated Linoleic Acid on Adipocyte Differentiation of Ovine and 3T3-L1 Preadipocytes
trans-10 cis-12 CLA Adipocyte Sheep
2016/5/3
Trans-10, cis-12 conjugated linoleic acid (CLA) has been reported to inhibit the adipocyte differentiation of preadipocytes in non-ruminant animals (mice, rat, and human). However, the effects of tran...
小鼠3T3-L1前脂肪细胞系的特异性标记(英文)
增强绿色荧光蛋白 脂肪组织脂肪酸结合蛋白 标记 细胞系
2008/1/2
摘要 用增强绿色荧光蛋白特异性标记小鼠 3T3 L1前脂肪细胞系 .构建paP2 promoter EGFP载体 ,电穿孔转染小鼠 3T3 L1前脂肪细胞 ,显微荧光观察和RT PCR确认aP2基因的内源表达 .EGFP基因转入 3T3 L1前脂肪细胞 ,观察到细胞分化过程中EGFP表达和脂肪积累 .RT PCR分析表明 ,EGFP代表了稳定而真实的aP2基因的内源性表达 .建立了由脂肪组织特异...