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Membrane fusion consists of a complex rearrangement of lipids and proteins that results in the merger of two lipid bilayers. We have developed a model system that employs synthetic DNA-lipid conjugate...
We recently described a strategy to prepare DNA-tethered lipid membranes either to fixed DNA on a surface or to DNA displayed on a supported bilayer [Boxer et al., J. Struct. Biol., 2009, 168, 190; Bo...
We recently introduced two approaches for tethering planar lipid bilayers as membrane patches to either a supported lipid bilayer or DNA-functionalized surface using DNA hybridization (Chung, M.; Lowe...
Specific membrane interactions such as lipid vesicle docking and fusion can be mediated by synthetic DNA-lipid conjugates as a model for the protein-driven processes that are ubiquitous in biological ...
Membrane-membrane recognition and binding are crucial in many biological processes. We report an approach to studying the dynamics of such reactions by using DNA-tethered vesicles as a general scaffol...
Lateral variations in membrane composition are postulated to play a central role in many cellular events, but it has been difficult to probe membrane composition and organization on length scales of t...
A simple and practical method for patterning supported lipid bilayers on poly(dimethylsiloxane) is presented. By using electron microscopy grids to laterally control the extent of plasma oxidation, th...
We report a new system of laterally mobile, arrayed vesicles that are encoded with DNA to control tethering to fluid-supported phospholipid bilayers. The motion of individual fluorescently labeled ves...
Phospholipid vesicles exhibit a natural tendency to fuse and assemble into a continuous single bilayer membrane on silica and several other substrate materials. The resulting supported membrane mainta...
Fluid-supported lipid bilayers self-assemble on glass and SiO2 surfaces. We have found that it is also possible to assemble fluid bilayers on plasma-oxidized polydimethyl siloxane (PDMS) surfaces. Fur...
Solid-supported lipid membranes are important for their roles in fundamental biophysical research as well as in applications such as biosensors. In our study, lipopolymers containing alkyl side chains...
The mechanism by which vesicles spontaneously form supported lipid bilayer membranes on glass surfaces is becoming better understood and this knowledge is the basis of a technology of patterning membr...
Virtually all structures in living cells are defined by lipid bilayers. Many molecular components can diffuse along these 2-dimensional structures, sampling all or part of the membrane, and this diffu...
In a fluid medium there is continuous diffusive mixing among components. In order to retain spatial information such as a concentration gradient or an image in the absence of external forces, the flui...
Two new methods are introduced for patterning fluid lipid bilayer membranes on solid supports. These methods are based on the observation that supported membranes undergo self-limiting lateral expansi...

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