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Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography
recombinant antigen ELISA immunoblo
2015/1/20
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal bindi...
BORONIC ACIDS AS LIGANDS FOR AFFINITY CHROMATOGRAPHY
boronic acids borate boronate boronate affinity chromatography separation of glycoproteins
2007/12/13
A review on the principles and applications of boronic acids as affinity ligands for the chromatographic separation of carbohydrates, nucleic acid components, glycoproteins, and other small biomolecul...