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旨在分析探讨TLR5基因启动子区甲基化修饰对断奶仔猪F18大肠杆菌抗性的调控作用。本研究首先利用qPCR和Western blot检测分析了TLR5基因在F18大肠杆菌抗性型和敏感型苏太断奶仔猪小肠组织（十二指肠和空肠）中的差异表达，然后利用生物信息学分析和双荧光素酶报告系统检测确定TLR5基因核心启动子区、CpG岛及其作用元件，进而检测并分析TLR5基因启动子区甲基化修饰与TLR5基因在F18大...
Effect of Two Doses of Different Zinc Sources (Inorganic vs. Chelated form) on the Epithelial Proliferative Activity and the Apoptotic Index of Intestinal Mucosa of Early-weaned Pigs Orally Challenged with E. coli K88
Weaning Pig Zinc Oxide Small Intestine Apoptosis Mitosis
2016/5/4
The effect of two doses of different sources of zinc, inorganic (zinc oxide) or chelated (zinc glutamate chelate), on morphology and turn-over of the small intestine was assessed in early-weaned pigs ...
Anti Microbial Resistance Profile of E. coli isolates From Tropical Free Range Chickens
E. coli Anti-microbial resistance Local fowl Turkey Roosters Cockerels Nigeria
2015/7/29
Normal intestinal flora of humans and animals constitute enormous reservoir of resistance genes for potentially pathogenic bacteria and may serve as major indictors of selection pressure exerted by an...
Assessing the Importance of Sand as a Source of Fecal Indicator Bacteria (Escherichia coli and Enterococcus)
Escherichia coli Enterococcus
2015/7/21
The purpose of this activity is for students to investigate water quality, and the factors that affect it, at a local beach. This is accomplished by (1) measuring concentrations of Escherichia coli an...
Replication restart in Escherichia coli: Genetic studies at the interface of recombination and replication
DNA replication forks DNA damage proteins cells enzyme catalysis
2015/1/28
DNA replication forks are frequently detained during their progression from oriC to the replication terminus due to encounters with DNA lesions or proteins blocking the way. For survival, cells deploy...
Oxidative stress responses in Escherichia coli and Pseudomonas aeruginosa
Aerobic respiration reactive oxygen species ROS damage biological molecules cells detoxification oxidative stress transcription factors signal differential gene expression escherichia coli pseudomonas aeruginosa
2015/1/28
Aerobic respiration generates unavoidable reactive oxygen species (ROS, detrimental by-products that damage biological molecules. The accumulation of ROS beyond the cellular capacity for detoxificatio...
Genetic studies of replication restart in Escherichia coli
DNA the genetic biological DNA damage protein chromosomes proteins to repair
2015/1/28
Faithful DNA replication is essential for all organisms to maintain genetic integrity. During the DNA replication, replications forks are frequently stalled or collapsed due to the encounter of DNA le...
The SOS response in Escherichia coli: Single cell analysis using fluorescence microscopy
Biological sciences DNA repair RecA SOS response
2015/1/28
During the course of DNA replication, replication forks often stall or collapse as they proceed from oriC to the terminus due to housekeeping types of DNA damage or proteins bound to DNA. If the DNA i...
Reca Dynamics & the SOS Response in Escherichia Coli: Cellular Limitation of Inducing Filaments
DNA damage RadA RecA SOS response
2015/1/28
During the course of normal DNA replication, replication forks are constantly encountering "housekeeping" types of routine damage to the DNA template that may cause the forks to stall or collapse. One...
Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography
recombinant antigen ELISA immunoblo
2015/1/20
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal bindi...
Gene typing of the colonisation factor K88 (F4) in enterotoxigenic Escherichia coli strains isolated from diarrhoeic piglets
K88 F4 PCR colonisation factor
2015/1/20
More than 4 000 E. coli strains isolated from diarrhoeic piglets in 111 pig herds in the Czech Republic during the period 1995–2000 were examined for serogroup and virulence factors. Gene typing of th...
Inhibition of protein synthesis in Escherichia coli by expression of RNAs containing multiple ribosome binding sites
To get together the biosynthesis of chemical biological synthesis mRNA
2014/12/11
Biologically synthesized poly(α,L-glutamic acid) (PLGA) can be chemically modified to form monodisperse poly(γ-benzyl α,L-glutamate) (PBLG). This material shows rare smectic ordering where macromolecu...
Understanding the allosteric mechanism of the Escherichia coli Hsp70 molecular chaperone, DnaK
Hsp70 Protein aggregation Enzyme Polypeptide Protein function Nucleotides. The ATP hydrolysis
2014/12/11
The Hsp70 family molecular chaperones prevent protein aggregation under heat shock conditions. They are highly conserved, and have a N-terminal ATPase domain, which binds and hydrolyzes ATP, and a C-t...
Genetic studies of the regulation of RecA activity in Escherichia coli: A two filament model
E. coli protein compound enzyme DNA repair bacteria
2014/12/11
The RecA protein of Escherichia coli is a central enzyme in recombination and SOS induction, both crucial DNA repair pathways. Furthermore, RecA and its homologues are found in all domains of life, fr...
Regulation of RecA-dependent homologous recombination by 3'-5' exonucleases and the UvrD helicase in Escherichia coli K-12
Cell repair DNA damage cell genetic cellular regulation molecular mechanisms
2014/12/11
Homologous recombination is generally considered a major mechanism by which cells repair many types of DNA lesions and damaged replication forks. However, if this process is left unchecked, cells ofte...