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为了解中甸刺玫(Rosa praelucens Byhouwer)异源十倍体的起源,以中甸刺玫及与其同域分布的 20 个蔷薇属近缘种为研究材料,以 45S rDNA 和 5S rDNA 为探针,采用双色荧光原位杂交(FISH)技术研究其核型。(1)21 种蔷薇中只有中甸刺玫的核型为 2B,其他种的核型均为 2A。(2)细胞分裂间期除拟木香的 45S rDNA 杂交位点与倍性不一致外,其他 20 种...
伴有多种FISH异常多发性骨髓瘤患者的预后分析
多发性骨髓瘤 荧光原位杂交 预后
2021/3/29
分析同时伴有2种及2种以上的荧光原位杂交(FISH)结果异常的多发性骨髓瘤(MM)患者的预后情况,筛选预后较差的MM者。 方法:共计纳入2011年7月至2017年2月期间767例在长征医院就诊的初诊MM患者,所有患者初诊时均进行FISH检测,所检测的FISH异常包括IgH易位、t(4;14)、t(11;14)、t(14;16)、17p-、1q扩增、-13/13q-,分析所有FISH异常对预后的影响...
Dual-color oligo-FISH can reveal chromosomal variations and evolution in Oryza species
Oryza species chromosomal variation dual-color oligo-FISH karyotype
2023/8/9
Fluorescence in situ hybridization using probes based on oligonucleotides (oligo-FISH) is a useful tool for chromosome identification and karyotype analysis. Here we developed two oligo-FISH probes th...
CSI-FiSh: Efficient Isogeny based Signatures through Class Group Computations
Isogeny-based cryptography digital signature class group
2019/5/21
In this paper we report on a new record class group computation of an imaginary quadratic field having 154-digit discriminant, surpassing the previous record of 130 digits. This class group is central...
EC、FISH及BTA联合在肾盂癌鉴别诊断中的应用价值
脱落细胞学 荧光原位杂交技术 膀胱肿瘤抗原 肾盂癌
2020/6/9
观察脱落细胞学检查(EC)、荧光原位杂交技术(FISH)及膀胱肿瘤抗原(BTA)3种方法联合诊断肾盂癌的临床价值。方法回顾性分析保定市第二医院泌尿外科120例疑似肾盂癌患者临床病历资料,记录所有患者EC、FISH及BTA结果,以病理活检结果为金标准,分析单一或联合采用3种检查方法诊断肾盂癌的准确性,并绘制受试者工作曲线(ROC),比较单一或联合采用3种检查方法的价值。
对杂交三倍体泥鳅(4n♀×2n♂)的胚胎染色体进行了C带及染色体荧光原位杂交(FISH)分析,首次探讨了杂交三倍体泥鳅C带特征,为准确鉴别染色体提供依据,研究了核糖体5.8S+28S rDNA在杂交三倍体泥鳅胚胎中期染色体上的数目和位置。C带结果表明,杂交三倍体泥鳅的染色体C带包括臂端C带和着丝粒C带,没有发现臂间C带。M染色体只有1号染色体既有臂端C带又有着丝粒C带,但臂端C带均比着丝粒C带大,...
Tissue Fatty Acid Deposition, Plasma Lipid and Cytokine Profile in Pigs Fed a Diet with Fish Oil or Palm Oil
EPA DHA cholesterol IL-10 TNF-α cardiovascular diseases feed ration pig muscle
2018/3/5
The present study tested a hypothesis concerning a favourable effect of dietary fish oil on the tissue polyunsaturated
fatty acid (PUFA) deposition, and on plasma lipid and cytokine profile. Thirty-t...
Randomized controlled trial assessing the efficacy of a reusable fish-shaped iron ingot to increase hemoglobin concentration in anemic, rural Cambodian women
anemia Cambodia hemoglobin inflammation iron deficiency iron ingot Lucky Iron Fish randomized controlled trial serum ferritin women of reproductive age
2018/11/15
Background: Anemia affects 45% of women of childbearing age in Cambodia. Iron supplementation is recommended in populations in which anemia prevalence is high. However, there are issues of cost, distr...
建立拟斯卑尔脱山羊草的FISH核型,分析明确不同来源拟斯卑尔脱山羊草的FISH核型特点,比较不同拟斯卑尔脱山羊草及其与普通小麦的FISH核型差异。【方法】以荧光标记的寡核苷酸Oligo-pTa535和Oligo-pSc119.2为探针,利用荧光原位杂交(fluorescence in situ hybridization,FISH)技术分析pTa535和pSc119.2在不同拟斯卑尔脱山羊草、四倍...
A metabolomic study of biomarkers of meat and fish intake
chicken red meat processed meat fish metabolomics
2018/11/13
Background: Meat and fish intakes have been associated with various chronic diseases. The use of specific biomarkers may help to assess meat and fish intake and improve subject classification accordin...
Sex differences in the effect of fish-oil supplementation on the adaptive response to resistance exercise training in older people: a randomized controlled trial
aging exercise fatty acids muscle sarcopenia
2018/11/12
Background: Resistance exercise increases muscle mass and function in older adults, but responses are attenuated compared with younger people. Data suggest that long-chain n–3 polyunsaturated fatty ac...
Fish consumption, fish oils, and cardiovascular events: still waiting for definitive evidence
Fish consumption fish oils cardiovascular event
2018/12/14
Beginning in the late 1960s, landmark epidemiologic observations made in fish-eating populations from Greenland, Canada, Alaska, China, and Japan suggested an inverse association between the consumpti...
Fish consumption, fish oils, and cardiovascular events: still waiting for definitive evidence
Fish sh consumption fish oils cardiovascular events definitive evidenceconsumption
2018/12/18
Beginning in the late 1960s, landmark epidemiologic observations made in fish-eating populations from Greenland, Canada, Alaska, China, and Japan suggested an inverse association between the consumpti...
Predictors of failure of fish-oil therapy for intestinal failure–associated liver disease in children
cholestasis fish oil fish oil lipid emulsion intestinal failure intestinal failure–associated liver disease Omegaven parenteral fish oil
2018/12/14
Background: Parenteral fish-oil (FO) therapy is a safe and effective treatment for intestinal failure–associated liver disease (IFALD). Patients whose cholestasis does not resolve with FO may progress...
Limitations of the review and meta-analysis of fish and PUFA intake and mild-to-severe cognitive impairment risks: a dose-response meta-analysis of 21 cohort studies
Limitation meta-analysis of fish 21 cohort studies
2018/12/13
The recent meta-analysis by Zhang et al. (1) on fish and PUFA intake and the risk of mild-to-severe cognitive impairment raises several methodologic issues that have important implications for the int...