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Purification and Characterization of Carbonic Anhydrase from Human Erythrocyte Plasma Membrane
Carbonic anhydrase Plasma membrane sulfanilamide KSCN and NaN3
2009/6/29
Carbonic anhydrase is the basic enzyme in inhalation function. Untill now no research had been done to determine whether CA is in the human erythrocyte plasma membrane or not. Carbonic anhydrase (CA) ...
Isolation and characterization of the plasma membrane of rat cauda epididymal spermatozoa
Isolation characterization plasma membrane rat cauda epididymal spermatozoa
2009/2/23
Cauda epididymal rat spermatozoa were isolated by flushing the excised epididymis and the plasma membrane was detached by a nitrogen cavitation treatment (500 psi, 10 minutes equilibration at 4 C)...
Modification of the rat sperm flagellar plasma membrane during maturation in the epididymis
Modification rat sperm flagellar plasma membrane maturation epididymis
2009/2/23
Previously, we demonstrated that surface radiolabeling of rat epididymal spermatozoa by lactoperoxidase-catalyzed iodination reveals a major component with an apparent molecular weight of 26,000 to ...
Atrial natriuretic factor inhibits the phosphorylation of protein kinase C in plasma membrane preparations of cultured Leydig tumor cells
Atrial natriuretic factor protein kinase C Leydig tumor cells cGMP plasma membranes
2009/2/12
The peptide hormone atrial natriuretic factor (ANF) exerts its effect in a receptor-mediated fashion and the membrane-bound form of guanylate cyclase represents a biologically active ANF receptor;...
Sertoli cell plasma membrane polypeptides involved in spermatogenic cell-Sertoli cell adhesion
Sertoli cell spermatogenic cell-Sertoli cell adhesion
2009/2/10
This study concerns Sertoli cell-spermatogenic cell adhesive interactions in the seminiferous tubule. Sertoli cell surface polypeptides involved in germ cell-Sertoli cell adhesion were identified ...
Changes in motion characteristics, plasma membrane integrity, and acrosome morphology during cryopreservation of buffalo spermatozoa
plasma membrane integrity morphology cryopreservation of buffalo spermatozoa
2008/12/26
Motion characteristics, plasma membrane integrity, and acrosome morphology of buffalo spermatozoa after different stages of cryopreservation (ie, dilution, cooling to 4 degrees C, equilibration at 4 d...
Calpain and calpastatin are located between the plasma membrane and outer acrosomal membrane of cynomolgus macaque spermatozoa
Calpain calpastatin plasma membrane outer acrosomal membrane cynomolgus macaque spermatozoa
2008/12/25
Mammalian sperm must undergo an acrosome reaction prior to penetration of the zona pellucida and subsequent fusion with an oocyte. Sperm gain the capability to acrosome react after a period of capac...
Measurement of intracellular calcium concentration and plasma membrane potential in human spermatozoa using flow cytometry
Measurement intracellular calcium concentration plasma membrane potential human spermatozoa
2008/12/25
We report 2 novel approaches using flow cytometry to measure intracellular calcium concentration and plasma membrane potential in human spermatozoa. Both approaches have the potential to measure dif...
Deterioration of Plasma Membrane Is Associated With Activated Caspases in Human Spermatozoa
Annexin V immunomagnetic cell depletion human spermatozoa caspases electron microscopy
2008/12/19
Spermatozoa with deteriorated plasma membranes can be separated by magnetic-activated cell sorting (MACS) after binding superparamagnetic annexin V-conjugated microbeads (ANMBs) to membrane phosphati...