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Investigation of peptide nucleic acid fluorescence in situ hybridization for diagnosis of ventilator-associated pneumonia in bronchoalveolar lavage specimens.
Investigation of peptide nucleic acid fluorescence in situ hybridization for diagnosis of ventilator-associated pneumonia in bronchoalveolar lavage specimens.
Background: Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome i...
A comparative nonisotopic in situ hybridization (ISH) analysis was carried out for the detection of Bmp-4, Shh and Wnt-5a transcripts during mice odontogenesis from initiation to cap stage. Bmp-4 was ...
The purposes of this study were twofold, namely to evaluate: (1) the effect of hybridization on microtensile bond strength (μTBS) to dentin, and (2) the ultrastructure of the dentin-adhesive interface...
Multicolor or multiplex fluorescence in situ hybridization (M-FISH) imaging is a recently developed molecular cytogenetic diagnosis technique for rapid visualization of genomic aberrations at the chro...
Cancer is a multifactorial and multistep process. Conventional cytogenetics has become a major working field in cancer research. Chromosome rearrangements are recognized as critical in the pathogenesi...
HPV is an epitheliotropic virus, predominantly associated with human skin and mucosal lesions. In many studies, the presence of HPV types 6/11, 16/18 and 31/33/51 have been demonstrated in oral lesion...
Background and Aim: Prenatal diagnosis of chromosome abnormalities is usually performed on amniotic fluid samples. Aneuploidy screening by fluorescence in situ hybridization (FISH), although not an al...
Cancer chemotherapy-induced nausea and vomiting have been demonstrated to involve humoral as well as neuronal mechanisms. A leading role of serotonin (5-hydroxytryptamine, 5-HT) in these mechanisms ...
Semenogelin I and II (Sgl, Sgll) are two separate gene products of chromosome 20 with extensive (80%) identity in primary structure. They are mainly responsible for immediate gel formation of fres...
A retrospective study was carried out to determine the prevalence of sex-chromosome mosaicism among azoospermic men and to evaluate the feasibility of using fluorescence in situ hybridization (FISH)...
摘要为了探讨用荧光原位杂交技术(fluorescence in situ hybridization, FISH)检测卵巢癌细胞中性染色体拷贝数目异常的实验方法及其应用价值,收集18例新鲜卵巢癌组织标本,以Biotin标记的X染色体α-卫星DNA(pBamX7)探针与经处理的标本进行卵巢癌细胞核的原位杂交,分别用Avidin-FITC和Anti-avidin进行信号的检测与放大,PI复染。于Oly...

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