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Preparation of Epithelial and Stromal Cell Fractions from Immature Rat Prostatic Tissue Using Percoll Gradients
prostate androgens testosterone
2009/2/19
A method is described for the dispersion, isolation, and partial characterization of epithelial and stromal tissue cells from the rat ventral prostate. Viable epithelial and stromal cells have bee...
Sperm function and choice of preparation media：comparison of Percoll and Accudenz discontinuous density gradients
Sperm maturity and creatine kinase motile sperm yield sperm activation chiortetracycline
2009/2/16
We compared the sperm populations prepared by Accudenz (35-65%) and Percoll (40-80%) density gradients in 21 normospermic specimens (concentration, 53.6 +/- 3.8 x 10(6) sperm/ml; motility, 44.5 +/...
Separation of cryopreserved human semen using Sephadex columns, washing, or Percoll gradients
Sperm separation intrauterine insemination seminal plasma sublethal damage
2009/2/7
The following methods were evaluated for their ability to separate motile cryopreserved sperm from semen after thawing: single washing, Percoll separation followed by a single washing, and Sephad...
Characterization of functional Leydig cells after purification on a continuous gradient of percoll
functional Leydig cells purification continuous gradient of percoll
2009/2/6
Two human chorionic gonadotropin (hCG) responsive cells from rat testicular interstitium were previously isolated on a discontinuous gradient of Percoll. The light cells were non-steroidogenic and...
Marked Differences in Protamine Content and P1/P2 Ratios in Sperm Cells From Percoll Fractions Between Patients and Controls
Chromatin condensation male infertility Percoll gradients sperm chromatin sperm morphology sperm motility
2008/12/19
The various sperm cell types present in a simple ejaculate differ in their motility and morphology. However, little is known about the nuclear maturity of these sperm cells and their relationship wi...