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在益生菌L.caseiZhang高密度培养小试（3L）基础上,进行30L到150L逐级放大中试生产工艺的研究以确定规模化生产工艺。在优化的发酵工艺下,150L规模发酵菌体密度可达2.9×10^10cfu/mL,与小试水平无差异。采用origin7.5软件在logistic equation基础上建立L.caseiZhang的生长和葡萄糖代谢动力学模型,模型与试验值拟合良好,平均误差小于10%,能够...
Molecular cloning and characterisation of alpha subunit of H+-ATPase in Lactobacillus casei Zhang
H+-ATPase clone sequence Lactobacillus casei Zhang phylogenic trees
2014/3/10
The acid tolerance is an important property of Lactic acid bacteria as potential probiotics. H+-ATPase is considered a key gene in several bacteria with the ability of acid tolerance. We cloned and se...