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Cryopreservation of Spermatozoa and Artificial Insemination：Past, Present, and Future
cryopreservation semen quality bull semen human semen semen donors
2009/2/19
This review article is intended to provide an in-depth analysis of the principles and programs established, as well as problems overcome, that have led to a successful artificial insemination (AI) p...
In vitro sperm-binding assay to distinguish differences in populations of human sperm or damage to sperm resulting from cryopreservation
Sperm quality fresh versus frozen semen
2009/2/18
Annually, >1.3 million men are members of couples seeking help because of infertility. Semen from many of these men contains reasonable numbers of motile and normal sperm, but for a subset of indivi...
Extender components and surfactants affect boar sperm function and membrane behavior during cryopreservation
Extender components surfactants boar sperm function membrane behavior cryopreservation
2009/2/18
To determine how the individual components of extenders affected boar sperm function and membrane structure and to test a new surfactant's cryoprotective ability, boar sperm were cryopreserved in ...
Artificial insemination using cryopreserved semen is a common management tool of the contemporary livestock producer. However, cryopreservation is detrimental to sperm function and fertility, kill...
The emergence of specialized procedures for the acquisition, processing, and cryopreservation of epididymal and testicular sperm in connection with intracytoplasmic sperm injection
specialized procedures acquisition cryopreservation epididymal testicular sperm intracytoplasmic sperm
2009/2/18
Epididymal and testicular spermatozoa have been used widely for ICSI among men with obstructive azoospermia and NOA. New aspiration and biopsy techniques are available, and several sperm prepa...
The effects of antifreeze peptide III (AFP) and insulin transferrin selenium（ITS）on cryopreservation of chimpanzee（Pan troglodytes）spermatozoa
Semen acrosome CAMA FITC-PSA
2009/2/17
We investigated the effects of antifreeze peptides (AFP) and insulin transferrin selenium (ITS) on the motility and membrane integrity of chimpanzee (Pan troglodytes) spermatozoa after chilling (0...
Premature capacitation of bovine spermatozoa is initiated by cryopreservation
Premature capacitation bovine spermatozoa cryopreservation
2009/2/17
Poor motility and abnormal acrosomal morphology only partially explain the reduced fertility of cryopreserved bovine spermatozoa. To test the hypothesis that cryopreservation procedures (dilution,...
Continuous assessment of human spermatozoa viability during cryopreservation
Sperm cryomicroscopy fluorescent viability probes
2009/2/17
Cryomicroscopy has enabled direct observation of freezing and thawing of human spermatozoa. When used with a fluorescent viability kit, sperm membrane damage was not apparent down to temperatures of...
Cryopreservation reduces the ability of equine spermatozoa to attach to oviductal epithelial cells and zonae pellucidae in vitro
equine spermatozoa oviductal epithelial cells zonae pellucidae
2009/2/13
Two bioassays were used to evaluate the interaction of fresh and cryopreserved equine semen with oviductal epithelial cells (OEC) and with the zona pellucida (ZP). Split ejaculates were either store...
Cryopreservation extenders affect calcium flux in bovine spermatozoa during a temperature challenge
Milk egg yolk-Tris indo-1 acetoxymethyl ester cold
2009/2/13
Bovine spermatozoa are commercially cryopreserved by diluting the cells in media, known as extenders, followed by slow cooling and freezing. Previous work has shown that this process of cryopreser...
Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation
superoxide dismutase loss human sperm cells cryopreservation
2009/2/12
Earlier studies on human sperm cryodamage have shown that plasma membrane stress is the primary process and that phospholipid peroxidation in cryopreserved samples is not inhibited by addition of an...
Effect of cryoprotective additives and cryopreservation protocol on sperm membrane lipid
Sperm PAF PTX cryoprotection lipid peroxidation
2009/2/12
Sperm membrane damage during cryopreservation reduces the recovery of motile sperm. The present study investigates changes in sperm motility and membrane lipid peroxidation (LPO) in response to two ...
Cryopreservation of human semen. Comparison of cryopreservatives, sources of variability, and prediction of post-thaw survival
Post-thaw sperm donor insemination infertility
2009/2/10
Human semen was cryopreserved using Human Sperm Preservation Medium, TEST-Yolk buffer, or glycerol alone. Sperm characteristics for each specimen were measured before and after freezing to determine...
Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation
superoxide dismutase activity sublethal cryodamage human sperm cryopreservation
2009/2/10
Cryopreservation of human sperm, now generally required in donor insemination programs, adversely affects the sperm in terms of standard sperm evaluation parameters and fertilizing ability. The fr...
Cryopreservation of mammalian sperm：what we ask them to survive
cryoprotectant membrane reorganization membrane permeability membranes metabolism metabolic balance
2009/2/6
Techniques for freezing bull sperm developed over the past 40 years have not yielded protocols for preserving sperm from other species. Recent advances in our understanding of cell membrane structur...