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本试验旨在建立IFAT和间接ELISA方法作为微小隐孢子虫病诊断方法。应用DNA重组技术,构建原核表达载体pGEX-P23,经IPTG诱导表达出GST-P23融合蛋白,并进行纯化,将该重组融合蛋白作为包被抗原,建立ELISA诊断方法;同样构建重组载体pMG-P23,用电转化方法将其转入干酪乳杆菌中,称为重组活干酪乳杆菌(含pMG-P23),以此为已知抗原,建立IFAT诊断方法。应用ELISA和IF...
Survey on Seroprevalence of Anti-Toxoplasma Gondii Antibodies in Cattle in Tabriz(Iran)by IFAT
Seroprevalence toxoplasma cattle Tabriz Iran
2009/1/19
Toxoplasmosis is a zoonotic disease in various species of animals caused by a sporozoan parasite, Toxoplasma gondii. The disease occurs throughout the world including Iran. This disease is a common in...